Characterization of silk fibroin modified surface: a proteomic view of cellular response proteins induced by biomaterials.
Characterization of silk fibroin modified surface: a proteomic view of cellular response proteins induced by biomaterials.
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DOI：
10.1155/2014/209469
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发表时间：
2014
影响因子：
--
通讯作者：
Tyan YC
中科院分区：
文献类型：
--
作者：
Yang MH;Yuan SS;Chung TW;Jong SB;Lu CY;Tsai WC;Chen WC;Lin PC;Chiang PW;Tyan YC
本研究旨在探索丝素蛋白（SF）生物聚合物表面诱导细胞膜蛋白活化的途径。以成纤维细胞为实验模型，利用质谱分析系统评估生物高分子材料诱导细胞蛋白的反应。表面覆盖多壁碳纳米管（CNTs）和SF，以增加表面积，增强生物聚合物的粘附，促进细胞增殖速率。石英晶体微天平（QCM）的CNTs/SF电极上粘附的成纤维细胞数量大大超过其他表面。此外，通过蛋白质组学方法分析黏附成纤维细胞在生物聚合物表面的差异蛋白表达，表明CD44可能是一个关键蛋白。通过本研究，提出了基于质谱的蛋白质组学在生物聚合物细胞粘附性评价中的应用。
The purpose of this study was to develop the pathway of silk fibroin (SF) biopolymer surface induced cell membrane protein activation. Fibroblasts were used as an experimental model to evaluate the responses of cellular proteins induced by biopolymer material using a mass spectrometry-based profiling system. The surface was covered by multiwalled carbon nanotubes (CNTs) and SF to increase the surface area, enhance the adhesion of biopolymer, and promote the rate of cell proliferation. The amount of adhered fibroblasts on CNTs/SF electrodes of quartz crystal microbalance (QCM) greatly exceeded those on other surfaces. Moreover, analyzing differential protein expressions of adhered fibroblasts on the biopolymer surface by proteomic approaches indicated that CD44 may be a key protein. Through this study, utilization of mass spectrometry-based proteomics in evaluation of cell adhesion on biopolymer was proposed.
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影响因子：
1.9
作者：
Kasemo, B
通讯作者：
Kasemo, B
影响因子：
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作者：
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DOI：
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1998-03-01
影响因子：
4
作者：
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通讯作者：
Herrlich, P
DOI：
10.1163/156856208784089607
发表时间：
2008-01-01
影响因子：
3.6
作者：
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通讯作者：
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