Organ Growth and Intestinal Functions of Preterm Pigs Fed Low and High Protein Formulas With or Without Supplemental Leucine or Hydroxymethylbutyrate as Growth Promoters.
Organ Growth and Intestinal Functions of Preterm Pigs Fed Low and High Protein Formulas With or Without Supplemental Leucine or Hydroxymethylbutyrate as Growth Promoters.
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DOI：
10.3389/fnut.2021.687703
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发表时间：
2021
影响因子：
5
通讯作者：
Buddington KK
中科院分区：
文献类型：
--
作者：
Buddington RK;Yakimkova T;Adebiyi A;Chizhikov VV;Iskusnykh IY;Buddington KK
早产儿或小于胎龄儿 (SGA) 肠内营养支持的目标是实现正常生长和发育。然而，由于肠道不成熟，这一目标很难实现。我们的目标是确定出生体重、蛋白质摄入量和生长促进剂亮氨酸 (10 g/L) 或β-羟基-β-甲基丁酸钙 (HMB；1.1 g/L) 是否会影响肠道生长轨迹以及其他器官的功能和重量。早产猪在妊娠第 105 天（足月的 91%）分娩，并饲喂 6 或 7 天的等热量配方奶，这些配方奶的蛋白质含量不同（50 克或 100 克蛋白质/升），含或不含生长促进剂亮氨酸或 HMB。为了比较目的，在分娩后 12 小时内测量了 6 头低出生体重足月猪和 6 头平均出生体重猪的器官重量。对于出生体重较低的早产猪，肠道生长和总肠刷状缘膜糖酶对蛋白质水平和补充亮氨酸的反应幅度更大。对于饲喂低蛋白代乳品的猪，毛喉素刺激的近端小肠的氯离子分泌较低。整个小肠转运葡萄糖的能力（mmol/kg·天）对蛋白质水平、亮氨酸或 HMB 没有反应，并且在小型猪和大型猪之间没有差异。小体重足月猪和平均体重足月猪的相对器官重量相似，但有些与早产猪的相对器官重量不同，表明早产，并且本研究使用的护理标准改变了肠道和其他器官的发育轨迹。尽管亮氨酸是一种有效的通用生长促进剂，可以增强小型早产猪的肠道发育，但它并不能减轻神经发育受损的情况。我们使用早产猪作为相关临床前模型的研究结果表明，营养支持策略可以影响某些胃肠道特征的发育和其他器官的生长。
The goal of enteral nutritional support for infants born preterm or small for gestational age (SGA) is to achieve normal growth and development. Yet, this is difficult to achieve because of intestinal immaturity. Our objective was to determine if birth weight, protein intake, and the growth promoters leucine (10 g/L) or calcium-ß-hydroxy-ß-methylbutryate (HMB; 1.1 g/L) would affect trajectories of intestinal growth and functions and weights of other organs. Preterm pigs were delivered at gestational day 105 (91% of term) and fed for 6 or 7 days isocaloric formulas that differed in protein content (50 g or 100 g protein/L), with and without the growth promoters leucine or HMB. For comparative purposes organ weights were measured within 12 h after delivery for six term pigs of low and six of average birth weights. The responses of intestinal growth and total intestinal brush border membrane carbohydrases to protein level and supplemental leucine were of greater magnitude for preterm pigs of lower birth weight. Forskolin stimulated chloride secretion in the proximal small intestine was lower for pigs fed the low protein milk replacers. Capacities of the entire small intestine to transport glucose (mmol/kg-day) were not responsive to protein level, leucine, or HMB, and did not differ between small and large pigs. Relative organ weights of the small and average weight term pigs were similar, but some differed from those of the preterm pigs suggesting preterm birth and the standards of care used for this study altered the trajectories of development for the intestine and other organs. Although leucine is an effective generalized growth promoter that enhances gut development of small preterm pigs, it does not mitigate compromised neurodevelopment. Our findings using preterm pigs as a relevant preclinical model indicate nutrition support strategies can influence development of some gastrointestinal tract characteristics and the growth of other organs.
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影响因子：
2.9
作者：
Baker, Jack;Workman, Megan;Pearson, Osbjorn
通讯作者：
Pearson, Osbjorn
DOI：
10.1097/mpg.0000000000001707
发表时间：
2018-03-01
影响因子：
2.9
作者：
Buddington, Randal K.;Davis, Samantha L.;Buddington, Karyl K.
通讯作者：
Buddington, Karyl K.
影响因子：
3.3
作者：
Douglas, S. L.;Edwards, S. A.;Kyriazakis, I.
通讯作者：
Kyriazakis, I.
宫内生长受限通过 JamC/Pard3a 分子途径影响颗粒细胞的径向迁移，从而损害小脑发育。
影响因子：
5.3
作者：
Iskusnykh IY;Fattakhov N;Buddington RK;Chizhikov VV
通讯作者：
Chizhikov VV
影响因子：
1.3
作者：
Feldpausch, Julie A.;Jourquin, Jan;Ritter, Matthew J.
通讯作者：
Ritter, Matthew J.