Characterization of vertebrate cohesin complexes and their regulation in prophase.
Characterization of vertebrate cohesin complexes and their regulation in prophase.
复制标题
DOI：
10.1083/jcb.151.4.749
复制
发表时间：
2000-11-13
期刊：
影响因子：
--
通讯作者：
Peters JM
中科院分区：
文献类型：
--
作者：
Sumara I;Vorlaufer E;Gieffers C;Peters BH;Peters JM
在真核生物中，姐妹染色单体从合成时一直保持连接，直到它们在后期分离。这种内聚依赖于一种叫做内聚蛋白的蛋白质复合物。在出芽酵母中，后期促进复合体（APC）途径通过去除染色体上的黏结蛋白来启动后期。在脊椎动物中，黏结蛋白从已经处于前期的染色体中分离出来。为了研究它们的有丝分裂调控，我们从人细胞中纯化了两个14S黏结蛋白复合物。这两种复合物都含有SMC1、SMC3、SCC1以及酵母Scc3p的同源物SA1和SA2中的任意一种。SA1也是爪蟾14S黏结蛋白的一个亚基。这些复合物与PDS5相互作用，PDS5是一种蛋白质，其真菌同源物与染色体内聚、凝聚和重组有关。大部分含有SA1-和sa2的复合物和PDS5都是染色质相关的，直到它们从前期到末期都可溶解。在有丝分裂的爪蟾提取物中对这一过程的重建表明，内聚蛋白的解离既不依赖于细胞周期蛋白B蛋白的水解，也不依赖于APC的存在。在缺乏周期蛋白依赖性激酶1活性的情况下，内聚蛋白也能与染色质分离。这些结果表明，脊椎动物的黏结蛋白受一种不同于酵母中控制黏结蛋白的APC途径的新的前期途径调控。
In eukaryotes, sister chromatids remain connected from the time of their synthesis until they are separated in anaphase. This cohesion depends on a complex of proteins called cohesins. In budding yeast, the anaphase-promoting complex (APC) pathway initiates anaphase by removing cohesins from chromosomes. In vertebrates, cohesins dissociate from chromosomes already in prophase. To study their mitotic regulation we have purified two 14S cohesin complexes from human cells. Both complexes contain SMC1, SMC3, SCC1, and either one of the yeast Scc3p orthologs SA1 and SA2. SA1 is also a subunit of 14S cohesin in Xenopus. These complexes interact with PDS5, a protein whose fungal orthologs have been implicated in chromosome cohesion, condensation, and recombination. The bulk of SA1- and SA2-containing complexes and PDS5 are chromatin-associated until they become soluble from prophase to telophase. Reconstitution of this process in mitotic Xenopus extracts shows that cohesin dissociation does neither depend on cyclin B proteolysis nor on the presence of the APC. Cohesins can also dissociate from chromatin in the absence of cyclin-dependent kinase 1 activity. These results suggest that vertebrate cohesins are regulated by a novel prophase pathway which is distinct from the APC pathway that controls cohesins in yeast.
登录
查看更多内容
DOI：
10.1083/jcb.116.3.707
发表时间：
1992-02
期刊：
The Journal of cell biology
影响因子：
--
作者：
Hunt T;Luca FC;Ruderman JV
通讯作者：
Ruderman JV
影响因子：
10.5
作者：
CohenFix, O;Peters, JM;Koshland, D
通讯作者：
Koshland, D
影响因子：
64.5
作者：
Ciosk, R;Zachariae, W;Nasmyth, K
通讯作者：
Nasmyth, K
影响因子：
11.4
作者：
Funabiki, H;Kumada, K;Yanagida, M
通讯作者：
Yanagida, M
影响因子：
11.4
作者：
Brandeis, M;Hunt, T
通讯作者：
Hunt, T