MicroRNA750-3p Targets Processing of Precursor 7 to Suppress Rice Black-Streaked Dwarf Virus Propagation in Vector Laodelphax striatellus.
MicroRNA750-3p Targets Processing of Precursor 7 to Suppress Rice Black-Streaked Dwarf Virus Propagation in Vector Laodelphax striatellus.
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DOI：
10.3390/v16010097
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发表时间：
2024-01-08
期刊：
影响因子：
--
通讯作者：
Ji Y
中科院分区：
文献类型：
--
作者：
Wang H;Dong Y;Xu Q;Wang M;Li S;Ji Y
microRNA（miRNAs）是一类非编码RNA，作为RNA干扰途径的成员，在抗病毒感染中起着关键作用。近80%的植物病毒是通过昆虫载体传播的，然而，对昆虫载体的miRNAs与植物病毒的相互作用知之甚少。本文以水稻黑条矮缩病毒（RBSDV）和灰飞虱（Laodelphax striatellus）为模型，研究microRNA 750 - 3 p（miR 750 - 3 p）在病毒传播中的调控作用。我们的研究结果表明，Ls-miR 750 - 3 p在RBSDV感染的SBPH中下调，并且主要在SBPH的中肠中表达。注射miR 750 - 3 p agomir显著降低病毒蓄积，注射miR 750 - 3 p抑制剂Agomir-750- 3 p显著促进SBPH中的病毒蓄积，如使用RT-qPCR和Western印迹检测的。前体7（POP 7）（RNase P和RNase MRP的亚基）的加工使用双荧光素酶报告基因测定作为miR 750 - 3 p的一个靶标进行筛选、鉴定和验证。POP 7的敲除显著增加了RBSDV病毒在SBPH中的繁殖，然后增加了SBPH的病毒传播速率。综上所述，我们的数据表明miR 750 - 3 p靶向POP 7以抑制其昆虫载体中的RBSDV感染。这些结果丰富了POP 7在调节病毒感染宿主昆虫中的作用，并为miRNAs在植物病毒和昆虫载体相互作用中的功能提供了新的见解。
MicroRNAs (miRNAs) are non-coding RNAs, which, as members of the RNA interference pathway, play a pivotal role in antiviral infection. Almost 80% of plant viruses are transmitted by insect vectors; however, little is known about the interaction of the miRNAs of insect vectors with plant viruses. Here, we took rice black-streaked dwarf virus (RBSDV), a devastating virus to rice production in eastern Asia, and the small brown planthopper, (SBPH, Laodelphax striatellus) as a model to investigate the role of microRNA750-3p (miR750-3p) in regulating viral transmission. Our results showed that Ls-miR750-3p was downregulated in RBSDV-infected SBPH and predominately expressed in the midgut of SBPH. Injection with miR750-3p agomir significantly reduced viral accumulation, and the injection with the miR750-3p inhibitor, antagomir-750-3p, dramatically promoted the viral accumulation in SBPH, as detected using RT-qPCR and Western blotting. The processing of precursor 7 (POP7), a subunit of RNase P and RNase MRP, was screened, identified, and verified using a dual luciferase reporter assay as one target of miR750-3p. Knockdown of POP7 notably increased RBSDV viral propagation in SBPH and then increased the viral transmission rate by SBPH. Taken together, our data indicate that miR750-3p targets POP7 to suppress RBSDV infection in its insect vector. These results enriched the role of POP7 in modulating virus infection in host insects and shared new insight into the function of miRNAs in plant virus and insect vector interaction.
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影响因子：
5.6
作者：
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作者：
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病毒与昆虫肠道的相互作用
DOI：
10.1146/annurev-virology-091919-100543
发表时间：
2021-01-01
期刊：
ANNUAL REVIEW OF VIROLOGY, VOL 8
影响因子：
--
作者：
Ma, Enhao;Zhu, Yibin;Cheng, Gong
通讯作者：
Cheng, Gong
影响因子：
12.3
作者：
Aravind L;Iyer LM;Anantharaman V
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