Expression of the Na+/I- symporter (NIS) is markedly decreased or absent in gastric cancer and intestinal metaplastic mucosa of Barrett esophagus.
Expression of the Na+/I- symporter (NIS) is markedly decreased or absent in gastric cancer and intestinal metaplastic mucosa of Barrett esophagus.
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DOI：
10.1186/1471-2407-7-5
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发表时间：
2007-01-10
期刊：
影响因子：
3.8
通讯作者：
Carrasco N
中科院分区：
文献类型：
--
作者：
Altorjay A;Dohán O;Szilágyi A;Paroder M;Wapnir IL;Carrasco N
钠/碘同调体（NIS）是一种质膜糖蛋白，介导碘（I-）在甲状腺、哺乳期乳腺、唾液腺和胃中的转运。尽管NIS在健康和肿瘤甲状腺和乳腺组织中的表达和调控已被广泛研究，但对于NIS在健康和病变胃肠道中的表达和功能知之甚少。因此，我们通过免疫组织化学分析155个胃肠道组织样本和免疫印迹分析来自83例患者的17个胃肿瘤的NIS表达。在健康的胃肠道中，我们观察到NIS仅在胃黏液产生上皮细胞的基底外侧区域表达。在胃炎中，无论是否存在幽门螺杆菌，在这些细胞中都观察到NIS阳性染色。值得注意的是，NIS在胃癌中不存在，与其组织学类型无关。仅在胃肿瘤的直接附近，即在组织学完整的粘膜中检测到局灶性微弱的NIS表达，在远离肿瘤的地方，表达逐渐增强并呈线性。结缔组织和基底柱状化生的Barrett粘膜NIS染色阳性，而肠化生的Barrett粘膜NIS染色阴性。肠化胃息肉也未见NIS染色。在肠化或胃粘膜恶性转化的情况下，NIS的表达明显降低或缺失，这表明NIS可能是胃恶性肿瘤以及Barrett粘膜等癌前病变诊断和预后的重要肿瘤标志物，从而将NIS的医学意义扩展到甲状腺疾病之外。
The sodium/iodide symporter (NIS) is a plasma membrane glycoprotein that mediates iodide (I-) transport in the thyroid, lactating breast, salivary glands, and stomach. Whereas NIS expression and regulation have been extensively investigated in healthy and neoplastic thyroid and breast tissues, little is known about NIS expression and function along the healthy and diseased gastrointestinal tract. Thus, we investigated NIS expression by immunohistochemical analysis in 155 gastrointestinal tissue samples and by immunoblot analysis in 17 gastric tumors from 83 patients. Regarding the healthy Gl tract, we observed NIS expression exclusively in the basolateral region of the gastric mucin-producing epithelial cells. In gastritis, positive NIS staining was observed in these cells both in the presence and absence of Helicobacter pylori. Significantly, NIS expression was absent in gastric cancer, independently of its histological type. Only focal faint NIS expression was detected in the direct vicinity of gastric tumors, i.e., in the histologically intact mucosa, the expression becoming gradually stronger and linear farther away from the tumor. Barrett mucosa with junctional and fundic-type columnar metaplasia displayed positive NIS staining, whereas Barrett mucosa with intestinal metaplasia was negative. NIS staining was also absent in intestinalized gastric polyps. That NIS expression is markedly decreased or absent in case of intestinalization or malignant transformation of the gastric mucosa suggests that NIS may prove to be a significant tumor marker in the diagnosis and prognosis of gastric malignancies and also precancerous lesions such as Barrett mucosa, thus extending the medical significance of NIS beyond thyroid disease.
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影响因子：
5.5
作者：
LOGOTHETOPOULOS, J;SCOTT, RF
通讯作者：
SCOTT, RF
影响因子：
5.8
作者：
Bidart, JM;Lacroix, L;Schlumberger, M
通讯作者：
Schlumberger, M
影响因子：
3.2
作者：
Ajjan, RA;Kamaruddin, NA;Weetman, AP
通讯作者：
Weetman, AP
影响因子：
4.8
作者：
Smanik, PA;Ryu, KY;Jhiang, SM
通讯作者：
Jhiang, SM
影响因子：
4.9
作者：
Fite, A;Dykhuizen, R;Leifert, C
通讯作者：
Leifert, C