Base-modified DNA labeled by [Ru(bpy)(3)](2+) and [Os(bpy)(3)](2+) complexes: construction by polymerase incorporation of modified nucleoside triphosphates, electrochemical and luminescent properties, and applications.
Base-modified DNA labeled by [Ru(bpy)(3)](2+) and [Os(bpy)(3)](2+) complexes: construction by polymerase incorporation of modified nucleoside triphosphates, electrochemical and luminescent properties, and applications.
复制标题
[Ru(bpy)(3)](2 ) 和 [Os(bpy)(3)](2 ) 复合物标记的碱基修饰 DNA：通过聚合酶掺入修饰三磷酸核苷进行构建、电化学和发光特性以及应用。
作者：
M. Vrábel;P. Horáková;Hana Pivoňková;Ľubica Kalachová;H. Černocká;Hana Cahová;R. Pohl;Peter Šebest;L. Havran;Michal Hocek;M. Fojta
Modified 2'-deoxynucleoside triphosphates (dNTPs) bearing [Ru(bpy)(3)](2+) and [Os(bpy)(3)](2+) complexes attached via an acetylene linker to the 5-position of pyrimidines (C and U) or to the 7-position of 7-deazapurines (7-deaza-A and 7-deaza-G) have been prepared in one step by aqueous cross-couplings of halogenated dNTPs with the corresponding terminal acetylenes. Polymerase incorporation by primer extension using Vent (exo-) or Pwo polymerases gave DNA labeled in specific positions with Ru(2+) or Os(2+) complexes. Square-wave voltammetry could be efficiently used to detect these labeled nucleic acids by reversible oxidations of Ru(2+/3+) or Os(2+/3+). The redox potentials of the Ru(2+) complexes (1.1-1.25 V) are very close to that of G oxidation (1.1 V), while the potentials of Os(2+) complexes (0.75 V) are sufficiently different to enable their independent detection. On the other hand, Ru(2+)-labeled DNA can be independently analyzed by luminescence. In combination with previously reported dNTPs bearing ferrocene, aminophenyl, and nitrophenyl tags, the Os-labeled dATP has been successfully used for "multicolor" redox labeling of DNA and for DNA minisequencing.
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影响因子：
56.9
作者：
MURPHY, CJ;ARKIN, MR;BARTON, JK
通讯作者：
BARTON, JK
含荧光 1,10-菲咯啉的寡核苷酸可区分完美碱基配对和错配碱基配对。
影响因子：
5.2
作者：
Hurley,DennisJ;Seaman,SusanE;Mazura,JanC;Tor,Yitzhak
通讯作者：
Tor,Yitzhak
影响因子：
3.9
作者：
Gorodetsky, Alon A.;Barton, Jacqueline K.
通讯作者：
Barton, Jacqueline K.
含氧化还原活性金属的核苷酸：合成、可调性和酶促掺入 DNA。
影响因子：
15
作者：
Weizman,Haim;Tor,Yitzhak
通讯作者：
Tor,Yitzhak
与寡核苷酸结合的镍 (II) 配合物的 1H NMR 研究：一种区分 DNA 中金属配合物结合位置的新技术。
DOI：
10.1021/ic0348291
发表时间：
2003
期刊：
Inorganic chemistry.
影响因子：
--
作者：
Bhattacharya,PratipK;Lawson,HollyJ;Barton,JacquelineK
通讯作者：
Barton,JacquelineK