Serum GP73, a marker for evaluating progression in patients with chronic HBV infections.
Serum GP73, a marker for evaluating progression in patients with chronic HBV infections.
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DOI：
10.1371/journal.pone.0053862
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Li X
中科院分区：
文献类型：
--
作者：
Wei H;Li B;Zhang R;Hao X;Huang Y;Qiao Y;Hou J;Li X;Li X
本研究旨在探讨血清 GP73 在诊断慢性乙型肝炎病毒 (HBV) 感染患者显着纤维化中的作用。两个人群进行了注册。所有受试者均为慢性乙型肝炎病毒感染患者。第一批患者包括 761 名患者，他们接受了肝脏硬度测量；第二组包括 633 名患者，他们进行了肝活检，其中 472 名患者的 ALT 几乎正常。所有患者均接受血清GP73检测。体外观察GP73重组蛋白对HepG2细胞和LX2细胞的作用。结果显示，血清GP73浓度与肝脏硬度相关（r = 0.601）。 ROC曲线下面积为0.76。 GP73诊断显着纤维化（≥F2）的敏感性和特异性分别为62.81%、80.05%（截止值：76.6 ng/ml）。血清 GP73 浓度与纤维化分级显着相关（分别在 633 名和 472 名患者中，r = 0.32 和 0.35）。GP73 在诊断慢性 HBV 感染患者的 S2 方面具有惊人的性能。在 472 名 ALT 接近正常的患者中，GP73 对 S2 诊断的敏感性和特异性分别为 62.5% 和 80.0%，其中临界值设定为 82 ng/ml。 GP73重组蛋白在10-100 ng/ml浓度下可促进LX2细胞增殖。目前的结果表明，GP73可能是诊断慢性HBV感染患者显着纤维化的标志物，并且可能是纤维化的新贡献者。
This study was designed to investigate the role of serum GP73 for diagnosing significant fibrosis in patients with chronic hepatitis B virus (HBV) infections. Two populations were enrollment. All subjects were patients with chronic HBV infections. First population included 761 patients, who received liver stiffness measurement; the second population included 633 patients, who undertaken liver biopsy, in which 472 patients with nearly normal ALT. All patients received serum GP73 test. The effect of GP73 recombinant protein to HepG2 cells and LX2 cells were observed in vitro. Results showed that serum GP73 concentration is correlated with liver stiffness (r = 0.601). The area under ROC curve is 0.76. The sensitivity and specificity of GP73 for significant fibrosis (≥F2) diagnosis were 62.81%, 80.05% respectively (cut off: 76.6 ng/ml). Serum GP73 concentration was significantly correlated with the grading of fibrosis (r = 0.32, and 0.35, in 633 and 472 patients, respectively.) GP73 had a striking performance for diagnosing S2 in patients with chronic HBV infections. In 472 patients with nearly normal ALT, the sensitivity and specificity of GP73 for S2 diagnosis were 62.5% and 80.0% respectively, where the cut-off was set at 82 ng/ml. GP73 recombinant protein may prompt LX2 cells proliferation at the concentration 10–100 ng/ml. The present results indicated that GP73 may be a marker for diagnosing significant fibrosis in patients with chronic HBV infections, and may be a new contributor to fibrogensis.
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