Collection and comparative analysis of 1888 full-length cDNAs from wild rice Oryza rufipogon Griff. W1943.
Collection and comparative analysis of 1888 full-length cDNAs from wild rice Oryza rufipogon Griff. W1943.
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DOI：
10.1093/dnares/dsn018
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发表时间：
2008-10
期刊：
影响因子：
--
通讯作者：
Han B
中科院分区：
文献类型：
--
作者：
Lu T;Yu S;Fan D;Mu J;Shangguan Y;Wang Z;Minobe Y;Lin Z;Han B
栽培稻的cDNA和EST资源已被大量开发，而野生稻的cDNA资源却很少。本研究从普通野生稻（Oryza rufipogon Griff）中分离了1888个全长cDNA克隆，并进行了全序列测定。W1943进行野生稻与栽培稻的比较分析。在正常或冷处理条件下，从3周龄的叶片样品构建两个cDNA文库。同源性分析表明，野生稻与栽培稻的同源性在96.8%以上。莴苣日本日本晴基因组序列。然而，其中<22%与CV完全匹配。日本晴基因组序列。比较分析表明，O. rufipogon W1943与O.莴苣japonica比ssp.籼稻品种此外，通过检索水稻大规模平行特征测序数据库，还鉴定了17个新的水稻cDNA，并定义了41个推定的组织特异性表达基因。总之，这些FLcDNA克隆是进一步功能验证的资源，可广泛用于水稻生物学研究。
A huge amount of cDNA and EST resources have been developed for cultivated rice species Oryza sativa; however, only few cDNA resources are available for wild rice species. In this study, we isolated and completely sequenced 1888 putative full-length cDNA (FLcDNA) clones from wild rice Oryza rufipogon Griff. W1943 for comparative analysis between wild and cultivated rice species. Two cDNA libraries were constructed from 3-week-old leaf samples under either normal or cold-treated conditions. Homology searching of these cDNA sequences revealed that >96.8% of the wild rice cDNAs were matched to the cultivated rice O. sativa ssp. japonica cv. Nipponbare genome sequence. However, <22% of them were fully matched to the cv. Nipponbare genome sequence. The comparative analysis showed that O. rufipogon W1943 had greater similarity to O. sativa ssp. japonica than to ssp. indica cultivars. In addition, 17 novel rice cDNAs were identified, and 41 putative tissue-specific expression genes were defined through searching the rice massively parallel signature-sequencing database. In conclusion, these FLcDNA clones are a resource for further function verification and could be broadly utilized in rice biological studies.
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影响因子：
64.8
作者：
Matsumoto, T;Wu, JZ;Sugiura, M
通讯作者：
Sugiura, M
影响因子：
5.4
作者：
Tian, Feng;Zhu, Zuofeng;Sun, Chuan Qing
通讯作者：
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影响因子：
6.2
作者：
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通讯作者：
Shin, JS
DOI：
10.1073/pnas.0603152103
发表时间：
2006-06-20
影响因子：
11.1
作者：
Londo, Jason P.;Chiang, Yu-Chung;Schaal, Barbara A.
通讯作者：
Schaal, Barbara A.
影响因子：
14.9
作者：
Griffiths-Jones S;Moxon S;Marshall M;Khanna A;Eddy SR;Bateman A
通讯作者：
Bateman A