Endogenous transcription at the centromere facilitates centromere activity in budding yeast.
Endogenous transcription at the centromere facilitates centromere activity in budding yeast.
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DOI：
10.1016/j.cub.2011.08.056
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发表时间：
2011-10-25
期刊：
影响因子：
9.2
通讯作者：
Kitagawa, Katsumi
中科院分区：
文献类型：
--
作者：
Ohkuni, Kentaro;Kitagawa, Katsumi
着丝粒(CEN)DNA-着丝粒复合体是一种特殊的染色质结构，它介导染色体附着在纺锤体上，是高保真染色体分离所必需的。尽管动粒功能从芽生酵母到人类都是保守的，但与包括分裂酵母在内的其他真核生物相比，人们认为转录在芽生酵母的着丝粒功能中没有作用。我们在这里报道，着丝粒的转录促进了发芽酵母中着丝粒的活性。我们鉴定了CEN DNA上的转录产物，发现CBF1是CEN DNA转录所必需的，CBF1是一种与CEN DNA结合的转录因子。CBF1Δ细胞的染色体不稳定性是由人工启动子驱动的转录抑制的。此外，我们通过体外动粒组装系统鉴定了转录因子STE12和STE12抑制因子Dig1是一个新的CEN相关蛋白复合体。Dig1在着丝粒抑制STE12依赖的转录。我们的研究表明，着丝粒的转录在芽期酵母着丝粒功能中起着重要的作用。
The centromere (CEN) DNA-kinetochore complex is the specialized chromatin structure that mediates chromosome attachment to the spindle and is required for high-fidelity chromosome segregation. Although kinetochore function is conserved from budding yeast to humans, it was thought that transcription had no role in centromere function in budding yeast, in contrast to other eukaryotes including fission yeast. We report here that transcription at the centromere facilitates centromere activity in the budding yeast Saccharomyces cerevisiae. We identified transcripts at CEN DNA and found that Cbf1, which is a transcription factor that binds to CEN DNA, is required for transcription at CEN DNA. Chromosome instability of cbf1Δ cells is suppressed by transcription driven from an artificial promoter. Furthermore, we have identified Ste12, which is a transcription factor, and Dig1, a Ste12 inhibitor, as a novel CEN-associated protein complex by an in vitro kinetochore assembly system. Dig1 represses Ste12-dependent transcription at the centromere. Our studies reveal that transcription at the centromere plays an important role in centromere function in budding yeast.
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