Characterization and complete genome analysis of Bacillus velezensis CB6 revealed ATP synthase subunit α against foodborne pathogens
Characterization and complete genome analysis of Bacillus velezensis CB6 revealed ATP synthase subunit α against foodborne pathogens
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贝莱斯芽孢杆菌 CB6 的表征和完整基因组分析揭示了针对食源性病原体的 ATP 合酶亚基 α
DOI：
10.1007/s00203-020-02102-8
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发表时间：
2020-11
期刊：
影响因子：
--
通讯作者：
Lingcong Kong
中科院分区：
文献类型：
--
作者：
Haipeng Zhang;Guanyi Xu;Changsi Mao;Yuxuan Xu;Wenlong Dong;Muhammad Inam;Siyu Pan;Renge Hu;Yiming Wang;Yu Wang;Hongxia Ma;Lingcong Kong
鉴于食源性多重耐药菌对动物和人类的严重威胁，寻找有效的抗菌化合物一直是科学家们的重要课题。在这里，我们从长白山的土壤中鉴定出一种能够抑制金黄色葡萄球菌生长的细菌。NR基因组数据库分析和系统发育分析表明，菌株CB6属于绒毛芽孢杆菌。我们发现菌株CB6的粗提物对食源性致病菌具有广谱抗菌活性。此外，我们还发现粗提物经木瓜酶处理后失去了抗菌活性。采用硫酸铵沉淀法、Sephadex G-75凝胶过滤柱和高效液相色谱系统对菌株CB6进行纯化。LC-MS/MS分析表明，该抗菌化合物为蛋白三磷酸腺苷合成酶亚基α，相对分子质量为55.397 KDa。此外，我们还报道了菌株CB6的全基因组序列，它由一个独特的3,963,507个核苷酸的环状染色体组成，有3749个编码基因，G+C含量为46.53%。该基因组包含12个具有抗菌功能的基因簇，占全基因组的20.947以上。值得注意的是，在菌株CB6基因组中鉴定了编码ATP-1蛋白的氨基酸序列。除了这些发现，我们推测，ATP-1蛋白可能为次级代谢物提供能量，这反过来将提高次级代谢物的抗菌活性。所有这些重要特性使ATP-1成为未来开发新型抗菌药物和食品防腐剂的潜在候选者。
Given the serious threat of foodborne multidrug-resistant bacteria to animals and humans, finding an effective antibacterial compound has always been an important topic for scientists. Here, from the soil of Changbaishan, we have identified a bacterium that can inhibit the growth of Staphylococcus aureus. Nr genome database analysis and phylogenetic analysis showed that strain CB6 belongs to Bacillus velezensis. We found that the crude extract of strain CB6 has broad-spectrum antibacterial activity against foodborne pathogens. In addition, we showed that the crude extract loses antibacterial activity after treatment with papain. Next, strain CB6 was purified using ammonium sulfate precipitation, a Sephadex G-75 gel filtration column and high-performance liquid chromatography system (HPLC). Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis indicated that the antibacterial compound was the protein ATP synthase subunit α (ATP-1), with a molecular weight of 55.397 KDa. Moreover, we reported the complete genome sequence of strain CB6, which is composed of a unique circular 3,963,507 bp chromosome with 3749 coding genes and a G + C content of 46.53%. The genome contained 12 gene clusters with antibacterial functions, which constituted over 20.947% of the complete genome. Of note, the amino acid sequence encoding the ATP-1 protein in the strain CB6 genome was identified. In addition to these findings, we speculate that the ATP-1 protein may provide energy for secondary metabolites, which in turn will improve the antibacterial activity of the secondary metabolites. All the above important features make the ATP-1 as a potential candidate for the development of new antibacterial drugs and food preservatives in the future.
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影响因子：
3.7
作者：
Devetter M;Háněl L;Řeháková K;Doležal J
通讯作者：
Doležal J
影响因子：
15.8
作者：
Havelaar AH;Kirk MD;Torgerson PR;Gibb HJ;Hald T;Lake RJ;Praet N;Bellinger DC;de Silva NR;Gargouri N;Speybroeck N;Cawthorne A;Mathers C;Stein C;Angulo FJ;Devleesschauwer B;World Health Organization Foodborne Disease Burden Epidemiology Reference Group
通讯作者：
World Health Organization Foodborne Disease Burden Epidemiology Reference Group
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DOI：
10.1093/bioinformatics/btt509
发表时间：
2013-11-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Nawrocki EP;Eddy SR
通讯作者：
Eddy SR
影响因子：
3.7
作者：
Walker BJ;Abeel T;Shea T;Priest M;Abouelliel A;Sakthikumar S;Cuomo CA;Zeng Q;Wortman J;Young SK;Earl AM
通讯作者：
Earl AM
影响因子：
6.7
作者：
Daniel, Diane S.;Lee, Sui M.;Rahman, Sadequr
通讯作者：
Rahman, Sadequr