Expression of the immunoglobulin superfamily cell adhesion molecules in the developing spinal cord and dorsal root ganglion.
Expression of the immunoglobulin superfamily cell adhesion molecules in the developing spinal cord and dorsal root ganglion.
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DOI：
10.1371/journal.pone.0121550
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Yoshida Y
中科院分区：
文献类型：
--
作者：
Gu Z;Imai F;Kim IJ;Fujita H;Katayama Ki;Mori K;Yoshihara Y;Yoshida Y
属于免疫球蛋白超家族（IgSF）的细胞粘附分子通过神经系统不同区域的异亲或同亲相互作用控制突触特异性。在发育中的脊髓中，本体感觉神经元和运动神经元之间形成了精细特异性的单突触连接，然而，IgSF分子是否参与调节这一过程尚不清楚。为了确定IgSF分子是否影响感觉-运动回路中突触特异性的建立，我们通过原位杂交检测了157个IgSF基因在发育中的背根神经节（DRG）和脊髓中的表达。我们发现许多IgSF基因在发育中的小鼠DRG和脊髓的感觉和运动神经元中表达。例如，Alcam、Mcam和Ocam是由脊髓腹侧运动神经元的一个子集表达的。进一步的分析表明，Ocam在闭孔肌运动神经元中表达，而不是在股四头肌运动神经元中表达，这表明Ocam可能调节这些感觉-运动反射弧的感觉-运动特异性。电生理分析显示，Ocam突变小鼠闭孔和股四头肌运动神经元的单突触感觉-运动连接的突触特异性没有明显缺陷。由于Ocam+运动神经元的一个子集也表达Alcam，因此Alcam或其他功能冗余的IgSF分子可能在控制感觉-运动特异性方面补偿Ocam。综上所述，这些结果表明，在发育过程中，IgSF分子在感觉和运动神经元中广泛表达，并且Ocam和其他IgSF分子可能在控制感觉-运动回路的特异性方面具有冗余功能。
Cell adhesion molecules belonging to the immunoglobulin superfamily (IgSF) control synaptic specificity through hetero- or homophilic interactions in different regions of the nervous system. In the developing spinal cord, monosynaptic connections of exquisite specificity form between proprioceptive sensory neurons and motor neurons, however, it is not known whether IgSF molecules participate in regulating this process. To determine whether IgSF molecules influence the establishment of synaptic specificity in sensory-motor circuits, we examined the expression of 157 IgSF genes in the developing dorsal root ganglion (DRG) and spinal cord by in situ hybridization assays. We find that many IgSF genes are expressed by sensory and motor neurons in the mouse developing DRG and spinal cord. For instance, Alcam, Mcam, and Ocam are expressed by a subset of motor neurons in the ventral spinal cord. Further analyses show that Ocam is expressed by obturator but not quadriceps motor neurons, suggesting that Ocam may regulate sensory-motor specificity in these sensory-motor reflex arcs. Electrophysiological analysis shows no obvious defects in synaptic specificity of monosynaptic sensory-motor connections involving obturator and quadriceps motor neurons in Ocam mutant mice. Since a subset of Ocam+ motor neurons also express Alcam, Alcam or other functionally redundant IgSF molecules may compensate for Ocam in controlling sensory-motor specificity. Taken together, these results reveal that IgSF molecules are broadly expressed by sensory and motor neurons during development, and that Ocam and other IgSF molecules may have redundant functions in controlling the specificity of sensory-motor circuits.
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