SARS-CoV-2 breakthrough infection in vaccinees induces virus-specific nasal-resident CD8+ and CD4+ T cells of broad specificity.
SARS-CoV-2 breakthrough infection in vaccinees induces virus-specific nasal-resident CD8+ and CD4+ T cells of broad specificity.
复制标题
DOI：
10.1084/jem.20220780
复制
发表时间：
2022-10-03
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
鼻腔中的病毒特异性T细胞可能提供针对SARS-CoV-2的直接保护层。本研究仅在感染后的疫苗接种者体内检测到SARS-CoV-2特异性鼻腔驻留T细胞，凸显了鼻腔激发在感染部位形成抗病毒免疫的重要性。上呼吸道中的常驻T细胞对SARS-CoV-2感染细胞的快速识别可能为预防COVID-19提供重要的保护层。肠外SARS-CoV-2疫苗接种或感染是否诱导对不同SARS-CoV-2蛋白具有特异性的鼻腔驻留T细胞尚不清楚。我们从COVID-19疫苗接种者的鼻粘膜中分离T细胞，这些疫苗接种后经历了SARS-CoV-2感染（n = 34）或未经历SARS-CoV-2感染（n = 16），并分析了它们的表型，SARS-CoV-2特异性，功能和持久性。鼻腔驻留SARS-CoV-2特异性CD 8+和CD 4 + T细胞几乎只在经历SARS-CoV-2突破性感染的疫苗接种者中检测到。重要的是，通过疫苗接种引发的刺突特异性T细胞不会抑制对其他SARS-CoV-2蛋白特异性T细胞的诱导。鼻腔驻留T细胞反应持续≥140天，具有最小的减弱迹象。这些数据突出了病毒鼻攻击在原发感染部位形成SARS-CoV-2特异性抗病毒免疫的重要性，并进一步定义了SARS-CoV-2混合免疫的免疫学特征。
Virus-specific T cells in the nasal cavity might provide an immediate layer of protection against SARS-CoV-2. This study detected SARS-CoV-2–specific nasal-resident T cells in vaccinees only after infection, highlighting the significance of nasal challenge in the formation of antiviral immunity at the site of infection. Rapid recognition of SARS-CoV-2–infected cells by resident T cells in the upper airway might provide an important layer of protection against COVID-19. Whether parenteral SARS-CoV-2 vaccination or infection induces nasal-resident T cells specific for distinct SARS-CoV-2 proteins is unknown. We isolated T cells from the nasal mucosa of COVID-19 vaccinees who either experienced SARS-CoV-2 infection after vaccination (n = 34) or not (n = 16) and analyzed their phenotype, SARS-CoV-2 specificity, function, and persistence. Nasal-resident SARS-CoV-2–specific CD8+ and CD4+ T cells were detected almost exclusively in vaccinees who experienced SARS-CoV-2 breakthrough infection. Importantly, the Spike-specific T cells primed by vaccination did not suppress the induction of T cells specific for other SARS-CoV-2 proteins. The nasal-resident T cell responses persisted for ≥140 d, with minimal sign of waning. These data highlight the importance of viral nasal challenge in the formation of SARS-CoV-2–specific antiviral immunity at the site of primary infection and further define the immunological features of SARS-CoV-2 hybrid immunity.
登录
查看更多内容
影响因子：
24.8
作者：
Isho B;Abe KT;Zuo M;Jamal AJ;Rathod B;Wang JH;Li Z;Chao G;Rojas OL;Bang YM;Pu A;Christie-Holmes N;Gervais C;Ceccarelli D;Samavarchi-Tehrani P;Guvenc F;Budylowski P;Li A;Paterson A;Yue FY;Marin LM;Caldwell L;Wrana JL;Colwill K;Sicheri F;Mubareka S;Gray-Owen SD;Drews SJ;Siqueira WL;Barrios-Rodiles M;Ostrowski M;Rini JM;Durocher Y;McGeer AJ;Gommerman JL;Gingras AC
通讯作者：
Gingras AC
影响因子：
17.1
作者：
Collier AY;Brown CM;McMahan KA;Yu J;Liu J;Jacob-Dolan C;Chandrashekar A;Tierney D;Ansel JL;Rowe M;Sellers D;Ahmad K;Aguayo R;Anioke T;Gardner S;Siamatu M;Bermudez-Rivera L;Hacker MR;Madoff LC;Barouch DH
通讯作者：
Barouch DH
影响因子：
64.5
作者：
Rodda LB;Morawski PA;Pruner KB;Fahning ML;Howard CA;Franko N;Logue J;Eggenberger J;Stokes C;Golez I;Hale M;Gale M Jr;Chu HY;Campbell DJ;Pepper M
通讯作者：
Pepper M
SARS-COV-2抗原暴露历史塑造表型和记忆CD8（+）T细胞的特异性。
影响因子：
30.5
作者：
Minervina, Anastasia A.;Pogorelyy, Mikhail, V;Kirk, Allison M.;Crawford, Jeremy Chase;Allen, E. Kaitlynn;Chou, Ching-Heng;Mettelman, Robert C.;Allison, Kim J.;Lin, Chun-Yang;Brice, David C.;Zhu, Xun;Vegesana, Kasi;Wu, Gang;Trivedi, Sanchit;Kottapalli, Pratibha;Darnell, Daniel;McNeely, Suzanne;Olsen, Scott R.;Schultz-Cherry, Stacey;Estepp, Jeremie H.;McGargill, Maureen A.;Wolf, Joshua;Thomas, Paul G.
通讯作者：
Thomas, Paul G.
DOI：
10.1056/nejmoa2203965
发表时间：
2022-07-07
期刊：
The New England journal of medicine
影响因子：
--
作者：
通讯作者：
--