Presence of extracellular DNA in the Candida albicans biofilm matrix and its contribution to biofilms.
Presence of extracellular DNA in the Candida albicans biofilm matrix and its contribution to biofilms.
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DOI：
10.1007/s11046-009-9264-y
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发表时间：
2010-05
期刊：
影响因子：
5.5
通讯作者：
Oliveira, Rosario
中科院分区：
文献类型：
--
作者：
Martins, Margarida;Uppuluri, Priya;Thomas, Derek P.;Cleary, Ian A.;Henriques, Mariana;Lopez-Ribot, Jose L.;Oliveira, Rosario
DNA已被描述为细菌生物膜中细胞外基质（ECM）的结构组分。在白色念珠菌中，关于胞外DNA（eDNA）对生物膜基质和整体结构的贡献的知识很少。本工作检测了白色念珠菌生物膜ECM中eDNA的存在并定量其量，以及DNA酶处理和外源DNA的添加对白色念珠菌生物膜ECM的影响。白色念珠菌生物膜发育作为eDNA在生物膜发育中作用的指标。我们能够检测到从C.白色念珠菌生物膜在流动条件下形成，尽管检测到的eDNA的量根据生长条件而不同，特别是关于用于生长生物膜的培养基。实验用C.使用微量滴定板模型静态形成的白色念珠菌生物膜表明，外源DNA（>160 ng/ml）的添加增加了生物膜生物量，相反，DNA酶处理（>0.03 mg/ml）在生物膜形成的较晚时间点降低了生物膜生物量。我们提出的证据eDNA在C。白色念珠菌生物膜结构和形成，与eDNA是成熟C.白色念珠菌生物膜，并在生物膜结构的完整性和维护中起主导作用。
DNA has been described as a structural component of the extracellular matrix (ECM) in bacterial biofilms. In Candida albicans there is a scarce knowledge concerning the contribution of extracellular DNA (eDNA) to biofilm matrix and overall structure. This work examined the presence and quantified the amount of eDNA in C.albicans biofilm ECM and the effect of DNase treatment and the addition of exogenous DNA on C. albicans biofilm development as indicators of a role for eDNA in biofilm development. We were able to detect the accumulation of eDNA in biofilm ECM extracted from C. albicans biofilms formed under conditions of flow, although the quantity of eDNA detected differed according to growth conditions, in particular with regards to the medium used to grow the biofilms. Experiments with C. albicans biofilms formed statically using a microtiter plate model indicated that the addition of exogenous DNA (>160 ng/ml) increases biofilm biomass and, conversely, DNase treatment (>0.03 mg/ml) decreases biofilm biomass at later time points of biofilm development. We present evidence for the role of eDNA in C. albicans biofilm structure and formation, consistent with eDNA being a key element of the ECM in mature C. albicans biofilms and playing a predominant role in biofilm structural integrity and maintenance.
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影响因子：
5.5
作者：
Uppuluri, Priya;Chaturvedi, Ashok K.;Lopez-Ribot, Jose L.
通讯作者：
Lopez-Ribot, Jose L.
影响因子：
3.6
作者：
Allesen-Holm, M;Barken, KB;Tolker-Nielsen, T
通讯作者：
Tolker-Nielsen, T
影响因子：
4.4
作者：
Bellocchio, S;Montagnoli, C;Romani, L
通讯作者：
Romani, L
影响因子：
3.4
作者：
Thomas, Derek P.;Bachmann, Stefano P.;Lopez-Ribot, Jose L.
通讯作者：
Lopez-Ribot, Jose L.
影响因子：
5.8
作者：
Yordanov, M;Dimitrova, P;Ivanovska, N
通讯作者：
Ivanovska, N