Design of a simple model of Candida albicans biofilms formed under conditions of flow: development, architecture, and drug resistance.
Design of a simple model of Candida albicans biofilms formed under conditions of flow: development, architecture, and drug resistance.
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DOI：
10.1007/s11046-009-9205-9
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发表时间：
2009-09
期刊：
影响因子：
5.5
通讯作者：
Lopez-Ribot, Jose L.
中科院分区：
文献类型：
--
作者：
Uppuluri, Priya;Chaturvedi, Ashok K.;Lopez-Ribot, Jose L.
大多数医疗设备上的白色念珠菌生物膜暴露于体液流中，为真菌细胞提供水和营养。虽然已经对静态条件下体外生长的白色念珠菌生物膜进行了详尽的研究，但对于在持续补充营养物质的情况下形成的生物膜却并非如此。在这里，我们描述了一个简单的流动生物膜（FB）模型，可以使用大多数微生物实验室常用的材料轻松构建该模型。我们证明，与静态条件下生长的生物膜相比，使用这种流动系统形成的白色念珠菌生物膜表现出更高的结构复杂性。白色念珠菌生物膜在连续介质流下生长迅速，到 8 小时时表现出与 24 小时静态生长的生物膜相似的特征。生物量测量和显微镜观察进一步表明，孵育 24 小时后，FB 比静态条件下生长的生物膜厚 2 倍以上。显微镜分析显示，这些生物膜的表面极其致密且有皱纹，与 24 小时静态生物膜中常见的开放菌丝层不同。抗真菌药敏试验结果显示，FB中的白色念珠菌细胞对大多数临床使用的抗真菌药物表现出较高的耐药性。
Candida albicans biofilms on most medical devices are exposed to a flow of body fluids that provide water and nutrients to the fungal cells. While C. albicans biofilms grown in vitro under static conditions have been exhaustively studied, the same is not true for biofilms developed under continuous flow of replenishing nutrients. Here, we describe a simple flow biofilm (FB) model that can be built easily with materials commonly available in most microbiological laboratories. We demonstrate that C. albicans biofilms formed using this flow system show increased architectural complexity compared to biofilms grown under static conditions. C. albicans biofilms under continuous medium flow grow rapidly, and by 8 h show characteristics similar to 24 h statically grown biofilms. Biomass measurements and microscopic observations further revealed that after 24 h of incubation, FB were more than 2 fold thicker than biofilms grown under static conditions. Microscopic analyses revealed that the surface of these biofilms was extremely compact and wrinkled, unlike the open hyphal layer typically seen in 24 h static biofilms. Results of antifungal drug susceptibility tests showed that C. albicans cells in FB exhibited increased resistance to most clinically used antifungal agents.
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影响因子：
4.9
作者：
Bachmann, SP;VandeWalle, K;López-Ribot, JL
通讯作者：
López-Ribot, JL
影响因子：
4.9
作者：
Ramage, G;vande Walle, K;López-Ribot, JL
通讯作者：
López-Ribot, JL
影响因子：
2.2
作者：
Honraet, K;Goetghebeur, E;Nelis, HJ
通讯作者：
Nelis, HJ
影响因子：
--
作者：
García-Sánchez, S;Aubert, S;d'Enfert, C
通讯作者：
d'Enfert, C
影响因子：
--
作者：
Richard, ML;Nobile, CJ;Mitchell, AP
通讯作者：
Mitchell, AP