Transcription Factor RrANT1 of Rosa rugosa Positively Regulates Flower Organ Size in Petunia hybrida.
Transcription Factor RrANT1 of Rosa rugosa Positively Regulates Flower Organ Size in Petunia hybrida.
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DOI：
10.3390/ijms23031236
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发表时间：
2022-01-22
影响因子：
5.6
通讯作者：
Feng L
中科院分区：
文献类型：
--
作者：
Xu Y;Xing Y;Wei T;Wang P;Liang Y;Xu M;Ding H;Wang J;Feng L
花是许多植物产生精油的主要器官。原花产量和表皮分泌细胞数是影响精油产量的主要因素。以我国栽培玫瑰品种“平阴1号”为试材，研究了RrANT 1基因对花器官发育的影响。从玫瑰基因组中鉴定出18个具有双AP 2结构域的AP 2转录因子。RANT 1属于euANT。亚细胞定位结果表明，RrANT 1蛋白定位于细胞核内。RrANT 1在花托中的相对表达量在发育过程中高于花瓣中的相对表达量，但从发育初期到衰老期两者的相对表达量均下降。与开花期花瓣中的表达量相比，大蕾期花瓣中的表达量最高（~48.8），花托中的表达量最高（~102.5）。仅在花托中高表达（~39.4）。RrANT 1过表达显著增加矮牵牛花和叶的大小（~1.2），以及花鲜重（~30%）。过量表达植株的花瓣表皮细胞总数显著增加（>40%）。本研究表明，RrANT 1基因的过表达可以通过促进细胞增殖来增加花的大小和重量，为创造新的月季种质以提高月季和精油产量提供了依据。
The flower is the main organ that produces essential oils in many plants. The yield of raw flowers and the number of secretory epidermal cells are the main factors for essential oil production. The cultivated rose species “Pingyin 1” in China was used to study the effect of RrANT1 on floral organ development. Eighteen AP2 transcription factors with dual AP2 domains were identified from Rosa rugosa genome. RrANT1 belonged to euANT. The subcellular localization results showed that RrANT1 protein is localized in the nucleus. The relative expression level of RrANT1 in the receptacle is higher than that in petals in the developmental stages, and both decreased from the initial phase to senescence. Compared with the RrANT1 expression level in petals in the blooming stage, RrANT1 expression level was significant in petals (~48.8) and highest in the receptacle (~102.5) in the large bud stage. It was only highly expressed in the receptacle (~39.4) in the blooming period. RrANT1 overexpression significantly increased petunia flower and leaf sizes (~1.2), as well as flower fresh weight (~30%). The total number of epidermis cells in the petals of overexpressing plants significantly increased (>40%). This study concluded that RrANT1 overexpression can increase the size and weight of flowers by promoting cell proliferation, providing a basis for creating new rose germplasm to increase rose and essential oil yield.
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影响因子：
7.2
作者：
Krizek, Beth A.;Blakley, Ivory C.;Loraine, Ann E.
通讯作者：
Loraine, Ann E.
影响因子：
6.9
作者：
Juárez-Corona ÁG;de Folter S
通讯作者：
de Folter S
影响因子：
6.9
作者：
Krizek BA;Bantle AT;Heflin JM;Han H;Freese NH;Loraine AE
通讯作者：
Loraine AE
影响因子：
11.6
作者：
Klucher, KM;Chow, H;Fischer, RL
通讯作者：
Fischer, RL
影响因子：
5.1
作者：
Krizek, Beth A.;Eaddy, Marcie
通讯作者：
Eaddy, Marcie