The reduction of retinal autofluorescence caused by light exposure.
The reduction of retinal autofluorescence caused by light exposure.
复制标题
DOI：
10.1167/iovs.09-3643
复制
发表时间：
2009-12
影响因子：
4.4
通讯作者：
Williams DR
中科院分区：
文献类型：
--
作者：
Morgan JI;Hunter JJ;Merigan WH;Williams DR
我们以前已经表明，长时间暴露于568 nm的光在低于最大允许的曝光安全限制的水平产生视网膜损伤之前，视网膜色素上皮细胞（RPE）的自体荧光在体内的短暂减少。在这里，我们确定曝光功率和持续时间的影响如何结合联合收割机产生这种自发荧光减少，并找到导致可检测的自发荧光减少的最小曝光。使用荧光自适应光学扫描激光检眼镜对猕猴视网膜进行成像，以在体内分辨单个RPE细胞。将视网膜暴露于568 nm光，在对向0.5°的正方形上，能量范围为1 J/cm 2至788 J/cm 2，其中功率和持续时间独立变化。5 J/cm 2及更高的体内暴露导致自发荧光立即降低，随后完全自发荧光恢复（暴露≤ 210 J/cm 2）或永久性RPE细胞损伤（暴露≥ 247 J/cm 2）。对于2 J/cm 2及更低的暴露，未观察到显著的自发荧光降低。暴露功率和持续时间的互易性对于测试的暴露保持，这意味着传递到视网膜的总能量，而不是其在时间上的分布，决定了自发荧光减少的量。这种相互作用与光化学起源是一致的，这可能会也可能不会导致视网膜变性。实施用于将光递送到视网膜的安全方法需要更好地理解引起自体荧光减少的机制。最后，使用不会导致自体荧光可检测减少的光水平证明了RPE成像。
We have previously shown that long exposure to 568 nm light at levels below the maximum permissible exposure safety limit produces retinal damage preceded by a transient reduction in the autofluorescence of retinal pigment epithelial (RPE) cells in vivo. Here, we determine how the effects of exposure power and duration combine to produce this autofluorescence reduction and find the minimum exposure causing a detectable autofluorescence reduction. Macaque retinas were imaged using a fluorescence adaptive optics scanning laser ophthalmoscope to resolve individual RPE cells in vivo. The retina was exposed to 568 nm light over a square subtending 0.5° with energies ranging from 1 J/cm2 to 788 J/cm2, where power and duration were independently varied. In vivo exposures of 5 J/cm2 and higher caused an immediate decrease in autofluorescence followed by either full autofluorescence recovery (exposures ≤ 210 J/cm2) or permanent RPE cell damage (exposures ≥ 247 J/cm2). No significant autofluorescence reduction was observed for exposures of 2 J/cm2 and lower. Reciprocity of exposure power and duration held for the exposures tested, implying that the total energy delivered to the retina, rather than its distribution in time, determines the amount of autofluorescence reduction. That reciprocity holds is consistent with a photochemical origin, which may or may not cause retinal degeneration. The implementation of safe methods for delivering light to the retina requires a better understanding of the mechanism causing autofluorescence reduction. Finally, RPE imaging was demonstrated using light levels that do not cause a detectable reduction in autofluorescence.
登录
查看更多内容
影响因子：
4.1
作者：
Lois, N;Halfyard, AS;Fitzke, FW
通讯作者：
Fitzke, FW
影响因子：
--
作者：
CRUICKSHANKS, KJ;KLEIN, R;KLEIN, BEK
通讯作者：
KLEIN, BEK
DOI：
10.1073/pnas.0408892102
发表时间：
2005-04-05
影响因子：
11.1
作者：
Cideciyan, AV;Jacobson, SG;Aguirre, GD
通讯作者：
Aguirre, GD
影响因子：
4.2
作者：
Lois, N;Halfyard, AS;Fitzke, FW
通讯作者：
Fitzke, FW
影响因子：
13.7
作者：
Darzins, P;Mitchell, P;Heller, RF
通讯作者：
Heller, RF