Flexible colonoscopy in mice to evaluate the severity of colitis and colorectal tumors using a validated endoscopic scoring system.
Flexible colonoscopy in mice to evaluate the severity of colitis and colorectal tumors using a validated endoscopic scoring system.
复制标题
DOI：
10.3791/50843
复制
发表时间：
2013-10-16
期刊：
影响因子：
--
通讯作者：
Cominelli F
中科院分区：
文献类型：
--
作者：
Kodani T;Rodriguez-Palacios A;Corridoni D;Lopetuso L;Di Martino L;Marks B;Pizarro J;Pizarro T;Chak A;Cominelli F
现代内窥镜用于研究目的极大地促进了我们对胃肠道病理学的理解。特别是，实验内窥镜对于需要对单个实验动物进行重复评估的研究非常有用，例如评估慢性炎症性肠病和结直肠癌进展机制的研究。然而，不同研究中使用的方法差异很大。至少已经发布了三种针对小鼠结肠炎的内窥镜评分系统，并且已发布的用于评估结直肠肿瘤的方案未能解决伴随的结肠炎症的存在。这项研究开发并验证了一种可重复的内窥镜评分系统，该系统同时整合了炎症和肿瘤的评估。这种新颖的评分系统具有三个主要组成部分：1）评估结直肠炎症的范围和严重程度（基于肛周发现、肠壁透明度、粘膜出血和局灶性病变），2）定量记录肿瘤病变（网格图和条形图），3）根据十进制单位对临床病例的病理和研究相关性进行数字排序，并指定观察到的病变和内镜并发症的类别（十进制标识符）。本文提供的视频和手稿是按照 IACUC 批准的方案准备的，以便研究人员使用经过充分验证和高度可重复的内窥镜方法对自己的实验小鼠进行评分。
The use of modern endoscopy for research purposes has greatly facilitated our understanding of gastrointestinal pathologies. In particular, experimental endoscopy has been highly useful for studies that require repeated assessments in a single laboratory animal, such as those evaluating mechanisms of chronic inflammatory bowel disease and the progression of colorectal cancer. However, the methods used across studies are highly variable. At least three endoscopic scoring systems have been published for murine colitis and published protocols for the assessment of colorectal tumors fail to address the presence of concomitant colonic inflammation. This study develops and validates a reproducible endoscopic scoring system that integrates evaluation of both inflammation and tumors simultaneously. This novel scoring system has three major components: 1) assessment of the extent and severity of colorectal inflammation (based on perianal findings, transparency of the wall, mucosal bleeding, and focal lesions), 2) quantitative recording of tumor lesions (grid map and bar graph), and 3) numerical sorting of clinical cases by their pathological and research relevance based on decimal units with assigned categories of observed lesions and endoscopic complications (decimal identifiers). The video and manuscript presented herein were prepared, following IACUC-approved protocols, to allow investigators to score their own experimental mice using a well-validated and highly reproducible endoscopic methodology.
登录
查看更多内容
影响因子：
4.4
作者：
Wirtz, S;Becker, C;Neurath, MF
通讯作者：
Neurath, MF
影响因子：
2.2
作者：
Ravnic, Dino J.;Konerding, Moritz A.;Mentzer, Steven J.
通讯作者：
Mentzer, Steven J.
影响因子：
14.8
作者：
Becker, C.;Fantini, M. C.;Neurath, M. F.
通讯作者：
Neurath, M. F.
影响因子：
7.7
作者：
Banerjee, Subhas;Shen, Bo;Stewart, Leslie E.
通讯作者：
Stewart, Leslie E.
影响因子：
7.7
作者：
Hensley, Harvey H.;Merkel, Carrie E.;Clapper, Margie L.
通讯作者：
Clapper, Margie L.