Characterizing the flagellar filament and the role of motility in bacterial prey-penetration by Bdellovibrio bacteriovorus.
Characterizing the flagellar filament and the role of motility in bacterial prey-penetration by Bdellovibrio bacteriovorus.
复制标题
表征鞭毛细丝和运动在细菌猎物渗透中的作用。
DOI：
10.1111/j.1365-2958.2006.05081.x
复制
发表时间：
2006-04
影响因子：
3.6
通讯作者：
Sockett RE
中科院分区：
文献类型：
--
作者：
Lambert C;Evans KJ;Till R;Hobley L;Capeness M;Rendulic S;Schuster SC;Aizawa S;Sockett RE
掠食性细菌Bdellovibrio bacteriovorus通过单个极性鞭毛的旋转快速游动，该鞭毛由鞭毛单体的螺旋细丝组成，包含在膜鞘中，由基础运动复合体提供动力。蛭弧菌与细菌猎物发生碰撞、进入并在其内部复制，这一过程先前认为首先需要鞭毛运动，然后在猎物进入时鞭毛脱落。本研究表明，鞭毛并不总是在猎物进入后脱落，我们研究了B. bacteriovorus的6个fliC鞭毛蛋白基因，发现它们都是保守的，并在基因组菌株HD100和广泛研究的实验室菌株109J中表达。五个fliC基因单独失活后，突变的蛭弧菌仍能产生鞭毛，并且是可运动的和掠食性的。第6个fliC基因失活后，正常的鞭毛合成和运动被破坏，但鞭毛鞘仍然紊乱。我们发现，当直接应用于yfp标记的猎物细菌的草坪时，这种不运动的突变体仍然能够提前出现，这表明鞭毛运动对于猎物进入不是必需的，但对于在液体环境中有效地遇到猎物很重要。
The predatory bacterium Bdellovibrio bacteriovorus swims rapidly by rotation of a single, polar flagellum comprised of a helical filament of flagellin monomers, contained within a membrane sheath and powered by a basal motor complex. Bdellovibrio collides with, enters and replicates within bacterial prey, a process previously suggested to firstly require flagellar motility and then flagellar shedding upon prey entry. Here we show that flagella are not always shed upon prey entry and we study the six fliC flagellin genes of B. bacteriovorus, finding them all conserved and expressed in genome strain HD100 and the widely studied lab strain 109J. Individual inactivation of five of the fliC genes gave mutant Bdellovibrio that still made flagella, and which were motile and predatory. Inactivation of the sixth fliC gene abolished normal flagellar synthesis and motility, but a disordered flagellar sheath was still seen. We find that this non-motile mutant was still able to predate when directly applied to lawns of YFP-labelled prey bacteria, showing that flagellar motility is not essential for prey entry but important for efficient encounters with prey in liquid environments.
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DOI：
10.1007/bf00338097
发表时间：
1986-01-01
期刊：
MOLECULAR AND GENERAL GENETICS
影响因子：
--
作者：
ROGERS, M;EKATERINAKI, N;SHERRATT, D
通讯作者：
SHERRATT, D
影响因子：
3.2
作者：
VARON, M;SHILO, M
通讯作者：
SHILO, M
影响因子：
5.6
作者：
SOUTHERN, EM
通讯作者：
SOUTHERN, EM
影响因子：
3.5
作者：
VIEIRA, J;MESSING, J
通讯作者：
MESSING, J
影响因子：
5.6
作者：
KANTO, S;OKINO, H;YAMAGUCHI, S
通讯作者：
YAMAGUCHI, S