Map-based cloning and promoter variation analysis of the lobed leaf gene BoLMI1a in ornamental kale (Brassica oleracea L. var. acephala).
Map-based cloning and promoter variation analysis of the lobed leaf gene BoLMI1a in ornamental kale (Brassica oleracea L. var. acephala).
复制标题
观赏羽衣甘蓝 (Brassica oleracea L. var. acephala) 分叶基因 BoLMI1a 的图位克隆和启动子变异分析
DOI：
10.1186/s12870-021-03223-y
复制
发表时间：
2021-10-06
影响因子：
5.3
通讯作者：
Zhang Y
中科院分区：
文献类型：
--
作者：
Zhang B;Chen W;Li X;Ren W;Chen L;Han F;Fang Z;Yang L;Zhuang M;Lv H;Wang Y;Zhang Y
叶形是羽衣甘蓝的重要农艺性状。变种acephala）。虽然在羽衣甘蓝中已经报道了一些与叶片形状相关的基因，但叶片形状形成的详细机制仍不清楚。本文报道了羽衣甘蓝的一个裂叶性状，旨在分析该性状的遗传规律，并筛选出强候选基因。对羽衣甘蓝F2和BC 1群体的遗传分析表明，羽衣甘蓝的裂叶性状受一对显性基因控制，命名为BoL 1 -1（Brassica oleracea lobed-leaf）。通过进行全基因组重测序和连锁分析，BoLl-1基因被精细地定位到染色体C 09上的127-kb间隔，两侧是SNP标记SL 4和SL 6，遗传距离分别为0.6cM和0.6cM。基于该区间内基因的注释，预测拟南芥中的LMI 1（LATE MERISTEM IDENTITY 1）的直向同源基因Bo 9 g181710为BoLl-1的候选基因，并将其重命名为BoLMI 1a。与无裂叶亲本18 Q2515相比，有裂叶亲本18 Q2513中BoLMI 1a的表达水平显著更高。亲本等位基因的序列分析显示BoLMI 1a的编码序列中没有序列变异，而在亲本18 Q2513的BoLMI 1a启动子区域内鉴定出1737 bp缺失、92 bp插入和SNP。与BoLMI 1a特异性标记对应的启动子变异的验证分析显示，该变异仅存在于裂叶羽衣甘蓝自交系中。本研究鉴定了一个裂叶基因BoLMI 1a，该基因被精细定位到一个127 kb的片段。在BoLMI 1a的启动子区域中鉴定出三种变化。BoLMI 1a基因在两个亲本间的转录水平存在较大差异，为进一步研究羽衣甘蓝叶片形态形成的分子机制提供了新的思路。在线版本包含补充材料，可通过10.1186/s12870-021-03223-y获得。
Leaf shape is an important agronomic trait in ornamental kale (Brassica oleracea L. var. acephala). Although some leaf shape-related genes have been reported in ornamental kale, the detailed mechanism underlying leaf shape formation is still unclear. Here, we report a lobed-leaf trait in ornamental kale, aiming to analyze its inheritance and identify the strong candidate gene. Genetic analysis of F2 and BC1 populations demonstrate that the lobed-leaf trait in ornamental kale is controlled by a single dominant gene, termed BoLl-1 (Brassica oleracea lobed-leaf). By performing whole-genome resequencing and linkage analyses, the BoLl-1 gene was finely mapped to a 127-kb interval on chromosome C09 flanked by SNP markers SL4 and SL6, with genetic distances of 0.6 cM and 0.6 cM, respectively. Based on annotations of the genes within this interval, Bo9g181710, an orthologous gene of LATE MERISTEM IDENTITY 1 (LMI1) in Arabidopsis, was predicted as the candidate for BoLl-1, and was renamed BoLMI1a. The expression level of BoLMI1a in lobed-leaf parent 18Q2513 was significantly higher compared with unlobed-leaf parent 18Q2515. Sequence analysis of the parental alleles revealed no sequence variations in the coding sequence of BoLMI1a, whereas a 1737-bp deletion, a 92-bp insertion and an SNP were identified within the BoLMI1a promoter region of parent 18Q2513. Verification analyses with BoLMI1a-specific markers corresponding to the promoter variations revealed that the variations were present only in the lobed-leaf ornamental kale inbred lines. This study identified a lobed-leaf gene BoLMI1a, which was fine-mapped to a 127-kb fragment. Three variations were identified in the promoter region of BoLMI1a. The transcription level of BoLMI1a between the two parents exhibited great difference, providing new insight into the molecular mechanism underlying leaf shape formation in ornamental kale. The online version contains supplementary material available at 10.1186/s12870-021-03223-y.
登录
查看更多内容
影响因子：
10.7
作者：
Price MN;Dehal PS;Arkin AP
通讯作者：
Arkin AP
影响因子：
8.7
作者：
Kawakatsu Y;Sakamoto T;Nakayama H;Kaminoyama K;Igarashi K;Yasugi M;Kudoh H;Nagano AJ;Yano K;Kubo N;Notaguchi M;Kimura S
通讯作者：
Kimura S
影响因子：
11.6
作者：
Nikovics, Krisztina;Blein, Thomas;Laufs, Patrick
通讯作者：
Laufs, Patrick
观赏羽衣甘蓝叶形基因BoFL的精细定位与鉴定
影响因子：
5.4
作者：
Feng, Xin;Li, Xin;Zhu, Pengfang
通讯作者：
Zhu, Pengfang
影响因子：
9.2
作者：
Sicard, Adrien;Thamm, Anna;Lenhard, Michael
通讯作者：
Lenhard, Michael