4D biofabrication via instantly generated graded hydrogel scaffolds.
4D biofabrication via instantly generated graded hydrogel scaffolds.
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DOI：
10.1016/j.bioactmat.2021.05.021
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发表时间：
2022-01
影响因子：
18.9
通讯作者：
Alsberg E
中科院分区：
文献类型：
--
作者：
Ding A;Lee SJ;Ayyagari S;Tang R;Huynh CT;Alsberg E
形成分级生物材料以提供用于 4D 生物制造的变形支架，在复杂结构的制造和组织/器官再生关键动力学的重现方面具有广阔的前景。在这里，我们描述了使用用于 4D 生物制造的单材料或多材料一步制造策略轻松生成可调节且稳健的梯度。通过简单地将可光交联聚合物大分子单体、光引发剂（PI）、紫外线吸收剂和活细胞的混合溶液进行光交联，可以生成具有预编程变形的载有细胞的梯度水凝胶。使用各种紫外线吸收剂在各种聚合物中证明了梯度形成，包括聚乙二醇 (PEG)、藻酸盐和明胶衍生物，这些紫外线吸收剂的紫外线光谱与 PI 紫外线吸收光谱存在重叠。此外，这种简单有效的方法被用作通用平台，与其他水凝胶工程技术（例如光掩模辅助微加工、光图案化、离子转移印刷和3D生物打印）集成，以制造更先进的充满细胞的支架结构。最后，在 4D 骨样组织形成研究中证明了 4D 组织工程的概念验证。该策略的简单性及其多功能性为解决充满细胞的单组分水凝胶支架实现时间形状变化的障碍铺平了新的途径，并可能加快用于生物应用的 4D 生物制造结构的开发。开发了一种用于 4D 水凝胶生物制造的单材料一步梯度法。水凝胶内的梯度范围和方向可以轻松调整。该策略被证明在各种水凝胶系统中都是有效的。该系统与各种其他生物制造技术高度兼容。通过该系统，展示了 4D 骨样组织的形成。
Formation of graded biomaterials to render shape-morphing scaffolds for 4D biofabrication holds great promise in fabrication of complex structures and the recapitulation of critical dynamics for tissue/organ regeneration. Here we describe a facile generation of an adjustable and robust gradient using a single- or multi-material one-step fabrication strategy for 4D biofabrication. By simply photocrosslinking a mixed solution of a photocrosslinkable polymer macromer, photoinitiator (PI), UV absorber and live cells, a cell-laden gradient hydrogel with pre-programmable deformation can be generated. Gradient formation was demonstrated in various polymers including poly(ethylene glycol) (PEG), alginate, and gelatin derivatives using various UV absorbers that present overlap in UV spectrum with that of the PI UV absorbance spectrum. Moreover, this simple and effective method was used as a universal platform to integrate with other hydrogel-engineering techniques such as photomask-aided microfabrication, photo-patterning, ion-transfer printing, and 3D bioprinting to fabricate more advanced cell-laden scaffold structures. Lastly, proof-of-concept 4D tissue engineering was demonstrated in a study of 4D bone-like tissue formation. The strategy's simplicity along with its versatility paves a new way in solving the hurdle of achieving temporal shape changes in cell-laden single-component hydrogel scaffolds and may expedite the development of 4D biofabricated constructs for biological applications. A one-material one-step gradient method for 4D hydrogel biofabrication was developed. The gradient range and orientation within the hydrogels can be easily adjusted. The strategy was demonstrated to be effective in a variety of hydrogel systems. The system is highly compatible with a variety of other biofabrication technologies. With this system, 4D bone-like tissue formation was demonstrated.
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影响因子：
13.6
作者：
Hinton TJ;Jallerat Q;Palchesko RN;Park JH;Grodzicki MS;Shue HJ;Ramadan MH;Hudson AR;Feinberg AW
通讯作者：
Feinberg AW
影响因子：
9.5
作者：
Fusco, Stefano;Huang, Hen-Wei;Pane, Salvador
通讯作者：
Pane, Salvador
影响因子：
13.6
作者：
Cui, Haitao;Liu, Chengyu;Zhang, Lijie Grace
通讯作者：
Zhang, Lijie Grace
影响因子：
19
作者：
Han, Daehoon;Morde, Riddish S.;Lee, Howon
通讯作者：
Lee, Howon
DOI：
10.1093/annonc/mdl985
发表时间：
2006-06-01
期刊：
Annals of oncology : official journal of the European Society for Medical Oncology
影响因子：
--
作者：
Hamad, H M A
通讯作者：
Hamad, H M A