Sexual dimorphism in the fly brain.
Sexual dimorphism in the fly brain.
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DOI：
10.1016/j.cub.2010.07.045
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发表时间：
2010-09-28
期刊：
影响因子：
9.2
通讯作者：
Jefferis, Gregory S. X. E.
中科院分区：
文献类型：
--
作者：
Cachero, Sebastian;Ostrovsky, Aaron D.;Yu, Jai Y.;Dickson, Barry J.;Jefferis, Gregory S. X. E.
性别特异性行为可能源于大脑结构或功能的差异。在果蝇中，大约 2000 个神经元中雄性特有的无果亚型的作用似乎对于雄性求爱行为的许多方面都是必要和充分的。最初的工作发现这些 fru+ 神经元解剖二态性的证据有限。随后，报道了中脑 fru+ 神经元的三个离散的解剖学差异，但接线中性别差异的整体组织尚不清楚。对果蝇大脑结构差异的全球研究发现，雄性和雌性之间存在巨大的体积差异，主要是在较高级的大脑中心。与此同时，使用带有可抑制细胞标记的镶嵌分析对 fru+ 神经元进行饱和克隆分析，鉴定出 62 个在大脑中产生 fru+ 神经元的神经母细胞谱系。通过对男性和女性大脑的图像进行配准，发现了男性中 19 种新的二态性；这些高度集中在男性增大的高级大脑中枢。七个二态谱系也有女性特有的乔木。此外，神经索中 51 个 fru+ 谱系中至少有 5 个是二态性的。我们使用这些数据来预测超过 700 个潜在的二态神经连接位点。这些在侧角的三阶嗅觉神经元中特别丰富，我们通过在同一大脑中用不同颜色标记伙伴神经元，为二态性解剖连接提供了强有力的证据。我们的分析揭示了雄性和雌性果蝇大脑之间的接线和总体解剖结构存在显着差异。侧角的相互连接差异为雄性和雌性果蝇对性信息素的相反反应提供了合理的解释。 ► 果蝇的高级大脑中心在性别之间表现出巨大的体积差异 ► 62类无果神经元中的22类在大脑中具有性别特异性投射 ► 这些导致大脑中超过700个潜在的二态性连接位点 ► 嗅觉神经元的解剖差异可能会改变信息素处理
Sex-specific behavior may originate from differences in brain structure or function. In Drosophila, the action of the male-specific isoform of fruitless in about 2000 neurons appears to be necessary and sufficient for many aspects of male courtship behavior. Initial work found limited evidence for anatomical dimorphism in these fru+ neurons. Subsequently, three discrete anatomical differences in central brain fru+ neurons have been reported, but the global organization of sex differences in wiring is unclear. A global search for structural differences in the Drosophila brain identified large volumetric differences between males and females, mostly in higher brain centers. In parallel, saturating clonal analysis of fru+ neurons using mosaic analysis with a repressible cell marker identified 62 neuroblast lineages that generate fru+ neurons in the brain. Coregistering images from male and female brains identified 19 new dimorphisms in males; these are highly concentrated in male-enlarged higher brain centers. Seven dimorphic lineages also had female-specific arbors. In addition, at least 5 of 51 fru+ lineages in the nerve cord are dimorphic. We use these data to predict >700 potential sites of dimorphic neural connectivity. These are particularly enriched in third-order olfactory neurons of the lateral horn, where we provide strong evidence for dimorphic anatomical connections by labeling partner neurons in different colors in the same brain. Our analysis reveals substantial differences in wiring and gross anatomy between male and female fly brains. Reciprocal connection differences in the lateral horn offer a plausible explanation for opposing responses to sex pheromones in male and female flies. ► Drosophila higher brain centers show robust volume differences between the sexes ► 22 of 62 classes of fruitless neurons have sex-specific projections in the brain ► These lead to >700 sites of potentially dimorphic connectivity in the brain ► Anatomical differences in olfactory neurons likely alter pheromone processing
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影响因子：
16.2
作者：
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通讯作者：
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影响因子：
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FRANCESCHINI, N;HARDIE, R;KIRSCHFELD, K
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