Blunted neuronal calcium response to hypoxia in naked mole-rat hippocampus.
Blunted neuronal calcium response to hypoxia in naked mole-rat hippocampus.
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DOI：
10.1371/journal.pone.0031568
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Fall CP
中科院分区：
文献类型：
--
作者：
Peterson BL;Larson J;Buffenstein R;Park TJ;Fall CP
裸鼹鼠是高度群居的、严格意义上的地下啮齿类动物，它们生活在封闭的、长期缺氧的洞穴里，群居在一起。与其他哺乳动物的大脑切片相比，裸鼹鼠的大脑切片显示出对缺氧的极端耐受性，这表明在更低氧条件下突触传递的维持和缺氧去极化的潜伏期长三倍。决定细胞对缺氧反应是否可逆或是否导致细胞死亡的一个关键因素可能是细胞内钙浓度的升高。在本研究中，我们采用荧光成像技术测量了缺氧时海马CA1锥体细胞内钙的相对变化。我们发现，与实验室小鼠的切片相比，裸鼹鼠的切片在缺氧时的钙积累明显减少。新生儿（出生后第6天）和较大年龄组（出生后第20天）的情况都是如此。此外，尽管两种裸鼹鼠在老年时都表现出更多的钙积累，但老年裸鼹鼠的钙积累反应甚至比年轻小鼠要小。细胞内钙对缺氧的迟钝反应可能有助于裸鼹鼠神经元的极端缺氧耐受性。研究结果是根据一个普遍的假设进行讨论的，即一个非常漫长或停滞的发育过程可能使成年裸鼹鼠的大脑保持通常只在新生哺乳动物中看到的缺氧耐受性。
Naked mole-rats are highly social and strictly subterranean rodents that live in large communal colonies in sealed and chronically oxygen-depleted burrows. Brain slices from naked mole-rats show extreme tolerance to hypoxia compared to slices from other mammals, as indicated by maintenance of synaptic transmission under more hypoxic conditions and three fold longer latency to anoxic depolarization. A key factor in determining whether or not the cellular response to hypoxia is reversible or leads to cell death may be the elevation of intracellular calcium concentration. In the present study, we used fluorescent imaging techniques to measure relative intracellular calcium changes in CA1 pyramidal cells of hippocampal slices during hypoxia. We found that calcium accumulation during hypoxia was significantly and substantially attenuated in slices from naked mole-rats compared to slices from laboratory mice. This was the case for both neonatal (postnatal day 6) and older (postnatal day 20) age groups. Furthermore, while both species demonstrated more calcium accumulation at older ages, the older naked mole-rats showed a smaller calcium accumulation response than even the younger mice. A blunted intracellular calcium response to hypoxia may contribute to the extreme hypoxia tolerance of naked mole-rat neurons. The results are discussed in terms of a general hypothesis that a very prolonged or arrested developmental process may allow adult naked mole-rat brain to retain the hypoxia tolerance normally only seen in neonatal mammals.
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影响因子：
6.3
作者：
DESHPANDE, JK;SIESJO, BK;WIELOCH, T
通讯作者：
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影响因子：
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作者：
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DOI：
10.1016/0300-9629(79)90197-x
发表时间：
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DOI：
10.1073/pnas.93.18.9493
发表时间：
1996-09-03
影响因子：
11.1
作者：
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影响因子：
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作者：
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