Characterization, sequencing and comparative genomic analysis of vB_AbaM-IME-AB2, a novel lytic bacteriophage that infects multidrug-resistant Acinetobacter baumannii clinical isolates.
Characterization, sequencing and comparative genomic analysis of vB_AbaM-IME-AB2, a novel lytic bacteriophage that infects multidrug-resistant Acinetobacter baumannii clinical isolates.
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DOI：
10.1186/1471-2180-14-181
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发表时间：
2014-07-05
期刊：
影响因子：
4.2
通讯作者：
Tong Y
中科院分区：
文献类型：
--
作者：
Peng F;Mi Z;Huang Y;Yuan X;Niu W;Wang Y;Hua Y;Fan H;Bai C;Tong Y
随着广谱抗生素、免疫抑制剂和糖皮质激素的使用，多重耐药鲍曼不动杆菌（MDR-AB）已成为医院感染的主要病原菌。最近噬菌体疗法的复兴可能为对抗耐药细菌感染提供新的治疗策略。本研究中，我们分离到一株潜伏期短、爆发量小、能迅速清除宿主悬浮液的裂解性噬菌体vB_AbaM-IME-AB 2，并对其进行了鉴定和全基因组比较研究。分离的噬菌体vB_AbaM-IME-AB 2具有二十面体的头部，并显示类似于肌尾病毒科的形态。凝胶分离实验表明，噬菌体颗粒至少含有9条蛋白条带，分子量范围为15-100 kDa。vB_AbaM-IME-AB_2对宿主细胞的吸附时间为9 min，吸附率大于99%，吸附潜伏期短（20 min），爆发量小（62 pfu/cell）。在双层法中能形成清晰的噬斑，并在4小时内清除宿主的悬浮液。对vB_AbaM-IME-AB 2的全基因组进行了测序和注释，结果表明vB_AbaM-IME-AB 2的基因组是由43，665个核苷酸组成的双链DNA分子。基因组的G + C含量为37.5%，有82个推定的编码序列（CDS）。我们比较了所有已知的鲍曼不动杆菌噬菌体的特征和全基因组序列。目前已测序的鲍氏不动杆菌只有3株，与噬菌体vB_AbaM-IME-AB 2的覆盖率分别为65%、50%、8%。四种鲍曼不动杆菌的核苷酸比对显示，一些CDS是相似的，没有观察到显著的重排。然而，这些噬菌体菌株的某些部分是非同源的。vB_AbaM-IME-AB 2是一个新颖独特的A.鲍曼氏噬菌体这些发现表明了一个共同的祖先和微生物多样性和进化。明确其特征和基因有助于多药耐药A。鲍曼不动杆菌
With the use of broad-spectrum antibiotics, immunosuppressive drugs, and glucocorticoids, multidrug-resistant Acinetobacter baumannii (MDR-AB) has become a major nosocomial pathogen species. The recent renaissance of bacteriophage therapy may provide new treatment strategies for combatting drug-resistant bacterial infections. In this study, we isolated a lytic bacteriophage vB_AbaM-IME-AB2 has a short latent period and a small burst size, which clear its host’s suspension quickly, was selected for characterization and a complete genomic comparative study. The isolated bacteriophage vB_AbaM-IME-AB2 has an icosahedral head and displays morphology resembling Myoviridae family. Gel separation assays showed that the phage particle contains at least nine protein bands with molecular weights ranging 15–100 kDa. vB_AbaM-IME-AB2 could adsorb its host cells in 9 min with an adsorption rate more than 99% and showed a short latent period (20 min) and a small burst size (62 pfu/cell). It could form clear plaques in the double-layer assay and clear its host’s suspension in just 4 hours. Whole genome of vB_AbaM-IME-AB2 was sequenced and annotated and the results showed that its genome is a double-stranded DNA molecule consisting of 43,665 nucleotides. The genome has a G + C content of 37.5% and 82 putative coding sequences (CDSs). We compared the characteristics and complete genome sequence of all known Acinetobacter baumannii bacteriophages. There are only three that have been sequenced Acinetobacter baumannii phages AB1, AP22, and phiAC-1, which have a relatively high similarity and own a coverage of 65%, 50%, 8% respectively when compared with our phage vB_AbaM-IME-AB2. A nucleotide alignment of the four Acinetobacter baumannii phages showed that some CDSs are similar, with no significant rearrangements observed. Yet some sections of these strains of phage are nonhomologous. vB_AbaM-IME-AB2 was a novel and unique A. baumannii bacteriophage. These findings suggest a common ancestry and microbial diversity and evolution. A clear understanding of its characteristics and genes is conducive to the treatment of multidrug-resistant A. baumannii in the future.
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影响因子：
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作者：
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通讯作者：
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