Effect of housing arrangement on fecal-oral transmission of avian hepatitis E virus in chicken flocks.
Effect of housing arrangement on fecal-oral transmission of avian hepatitis E virus in chicken flocks.
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鸡群饲养安排对戊型肝炎病毒粪口传播的影响
DOI：
10.1186/s12917-017-1203-4
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发表时间：
2017-09-07
影响因子：
2.6
通讯作者：
Zhao Q
中科院分区：
文献类型：
--
作者：
Liu B;Sun Y;Chen Y;Du T;Nan Y;Wang X;Li H;Huang B;Zhang G;Zhou EM;Zhao Q
禽戊型肝炎病毒(HEV)感染在中国的鸡群中很常见，因为目前没有防止该疾病传播的措施。在这项研究中，我们分析了笼舍和非笼舍安排对禽类HEV传播的影响。首先，采集陕西省中国等4个鸡群的血清和临床粪便标本12 7份，检测戊型肝炎病毒抗体和病毒。同时，将36只无特定病原体的雏鸡随机分为4组，分别为无笼(接种)组、笼养(接种)组、非笼养(阴性)组和笼养(阴性)组。在笼养组，三个笼子每个笼子里装3只鸡。分别用相同剂量的禽戊型肝炎病毒(HEV)皮下尺静脉接种无笼(接种)组和笼(接种)组(每笼1只)各3只鸡。空笼(阴性)组和笼养(阴性)组作为阴性对照。在接种后1~7周(WPI)采集血清和粪便标本，并在接种后7周对肝脏病变进行评分。血清学检测结果表明，非笼养鸡的禽HEV感染率(54.10%)显著高于笼养鸡(12.12%)(P<0.05)。临床粪便标本中HEV RNA的检出率，无笼组(22.80%，13/57)显著高于笼养组(5.66%，3/53)。此外，在实验条件下，未接种笼养鸡(6只)的感染数量显著高于未接种笼养鸡(2只)，表现为血清阳转率、粪便病毒脱落、病毒血症以及大体和微观肝脏病变。这些结果表明，减少与粪便的接触，如在笼养鸡舍中所见，可以减少禽类HEV的传播。本研究为预防和控制鸡群禽HEV感染提供了新的思路。
Avian hepatitis E virus (HEV) infection is common in chicken flocks in China, as currently no measures exist to prevent the spread of the disease. In this study, we analyzed the effect of caged versus cage-free housing arrangements on avian HEV transmission. First, 127 serum and 110 clinical fecal samples were collected from 4 chicken flocks including the two arrangements in Shaanxi Province, China and tested for HEV antibodies and/or virus. Concurrently, 36 specific-pathogen-free chickens were divided equally into four experimental living arrangement groups, designated cage-free (Inoculated), caged (Inoculated), cage-free (Negative) and caged (Negative) groups. In caged groups, three cages contained 3 chickens each. Three chickens each from cage-free (Inoculated) and caged (Inoculated) groups (one chicken of each cage) were inoculated by cutaneous ulnar vein with the same dose of avian HEV, respectively. The cage-free (Negative) and caged (Negative) groups served as negative control. Serum and fecal samples were collected at 1 to 7 weeks post-inoculation (wpi) and liver lesions were scored at 7 wpi. The results of serology showed that the avian HEV infection rate (54.10%) of the cage-free chickens was significantly higher than the one (12.12%) for caged chickens (P < 0.05). Also, the rate of detection of avian HEV RNA in the clinical fecal samples was significantly higher in the cage-free (22.80%, 13/57) than caged birds (5.66%, 3/53). Moreover, under experimental conditions, the infected number of uninoculated cage-free chickens (6) was significantly higher than the one for the uninoculated caged birds (2), as evidenced by seroconversion, fecal virus shedding, viremia and gross and microscopic liver lesions. These results suggest that reduction of contact with feces as seen in the caged arrangement of housing chickens can reduce avian HEV transmission. This study provides insights for prevention and control of avian HEV infection in chicken flocks.
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影响因子：
11.8
作者：
Zhao Q;Zhou EM;Dong SW;Qiu HK;Zhang L;Hu SB;Zhao FF;Jiang SJ;Sun YN
通讯作者：
Sun YN
影响因子：
3.3
作者：
Meng XJ
通讯作者：
Meng XJ
禽戊型肝炎病毒 (HEV) 衣壳蛋白 N 末端区域中猪和人 HEV 不常见的抗原域的鉴定
影响因子：
3.8
作者：
Wang, Lizhen;Sun, Yani;Zhou, En-Min
通讯作者：
Zhou, En-Min
影响因子：
3.8
作者：
Haqshenas, G;Shivaprasad, HL;Meng, XJ
通讯作者：
Meng, XJ
影响因子：
2.2
作者：
Gerber, Priscilla F.;Trampel, Darrell W.;Opriessnig, Tanja
通讯作者：
Opriessnig, Tanja