Global identification and characterization of miRNA family members responsive to potassium deprivation in wheat (Triticum aestivum L.).
Global identification and characterization of miRNA family members responsive to potassium deprivation in wheat (Triticum aestivum L.).
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小麦（Triticum aestivum L.）缺钾反应的 miRNA 家族成员的整体鉴定和表征
DOI：
10.1038/s41598-020-72642-y
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发表时间：
2020-09-25
影响因子：
4.6
通讯作者：
Xiao K
中科院分区：
文献类型：
--
作者：
Zhao Y;Xu K;Liu G;Li S;Zhao S;Liu X;Yang X;Xiao K
钾（K）是植物生长和胁迫反应所必需的。microRNA（miRNAs）通过调节基因表达参与营养缺乏的适应。在此，我们基于高通量小RNA测序分析鉴定了小麦缺钾反应的miRNAs。89个miRNAs，包括68个已报道的miRNAs和21个新发现的miRNAs，在缺钾条件下表现出差异表达。在基因本体论和京都百科全书和基因组分析中，差异表达的miRNA的推定靶基因被分类为与ADP结合活性、次级代谢途径以及生物合成和代谢相关的功能组。tae-miR 408是一种在缺钾条件下显著下调的miRNA，其功能特性揭示了其在介导低钾耐受中的重要作用。与野生型相比，过量表达tae-miR 408的转基因烟草株系在缺钾条件下表现出显著提高的钾吸收、生物量、光合作用和活性氧清除。这些结果表明，不同的miRNAs通过调控参与能量代谢和多种次生代谢途径的靶基因参与植物对缺钾的响应。我们的发现揭示了T.小麦不同的miRNA，例如tae-miR 408，是产生具有提高钾利用效率的作物品种的有价值的靶点。
Potassium (K) is essential for plant growth and stress responses. MicroRNAs (miRNAs) are involved in adaptation to nutrient deprivation through modulating gene expression. Here, we identified the miRNAs responsive to K deficiency in Triticum aestivum based on high-throughput small RNA sequencing analyses. Eighty-nine miRNAs, including 68 previously reported ones and 21 novel ones, displayed differential expression under K deficiency. In Gene Ontology and Kyoto Encyclopedia and Genome analyses, the putative target genes of the differentially expressed miRNAs were categorized into functional groups associated with ADP-binding activity, secondary metabolic pathways, and biosynthesis and metabolism. Functional characterization of tae-miR408, an miRNA significantly down-regulated under K deficiency, revealed its important role in mediating low-K tolerance. Compared with wild type, transgenic tobacco lines overexpressing tae-miR408 showed significantly improved K uptake, biomass, photosynthesis, and reactive oxygen species scavenging under K deficiency. These results show that distinct miRNAs function in the plant response to K deficiency through regulating target genes involved in energy metabolism and various secondary metabolic pathways. Our findings shed light on the plant response to K deficiency mediated by miRNAs in T. aestivum. Distinct miRNAs, such as tae-miR408, are valuable targets for generating crop varieties with improved K-use efficiency.
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影响因子：
5.6
作者：
Driedonks N;Xu J;Peters JL;Park S;Rieu I
通讯作者：
Rieu I
影响因子：
5.1
作者：
Feuerbach, F;Lucas, H
通讯作者：
Lucas, H
影响因子：
5.1
作者：
Jia, Xiaoyun;Wang, Wang-Xia;Tang, Guiliang
通讯作者：
Tang, Guiliang
影响因子：
6.4
作者：
Gu, Mian;Xu, Ke;Xu, Guohua
通讯作者：
Xu, Guohua
影响因子：
16
作者：
Jones-Rhoades, MW;Bartel, DP
通讯作者：
Bartel, DP