Gram positive and Gram negative bacteria differ in their sensitivity to cold plasma.
Gram positive and Gram negative bacteria differ in their sensitivity to cold plasma.
复制标题
DOI：
10.1038/srep38610
复制
发表时间：
2016-12-09
影响因子：
4.6
通讯作者：
Murphy AB
中科院分区：
文献类型：
--
作者：
Mai-Prochnow A;Clauson M;Hong J;Murphy AB
冷常压等离子体(CAP)是一种相对较新的抗菌活性研究方法。然而，具体的行动模式仍在探索中。在这里，我们报告说，在几个物种中，CAP的疗效与细菌细胞壁厚度直接相关。革兰氏阳性枯草芽孢杆菌的生物膜具有55.4log10 nm的细胞壁，对环磷酰胺的抗性最强，10 处理后生物膜对环磷酰胺的抗性下降不到1log10。而革兰氏阴性铜绿假单胞菌只有2.4 nm的细胞壁，在相同的处理条件下，被膜几乎完全被清除。革兰氏阴性假单胞菌浮游培养的log10下降率也高于革兰氏阳性表皮葡萄球菌。铜绿假单胞菌和表皮葡萄球菌的混合生物膜表现出相似的趋势，即革兰氏阳性菌对CAP的耐药性更强。然而，当共培养时，革兰氏阴性铜绿假单胞菌对CAP的整体抵抗力强于单一物种生物被膜。发射光谱表明，等离子体中存在能够破坏胞壁结构键的OH和O。这项研究表明，细胞壁厚与细菌CAP失活时间有关，但细胞膜和生物膜基质也可能起作用。
Cold atmospheric-pressure plasma (CAP) is a relatively new method being investigated for antimicrobial activity. However, the exact mode of action is still being explored. Here we report that CAP efficacy is directly correlated to bacterial cell wall thickness in several species. Biofilms of Gram positive Bacillus subtilis, possessing a 55.4 nm cell wall, showed the highest resistance to CAP, with less than one log10 reduction after 10 min treatment. In contrast, biofilms of Gram negative Pseudomonas aeruginosa, possessing only a 2.4 nm cell wall, were almost completely eradicated using the same treatment conditions. Planktonic cultures of Gram negative Pseudomonas libanensis also had a higher log10 reduction than Gram positive Staphylococcus epidermidis. Mixed species biofilms of P. aeruginosa and S. epidermidis showed a similar trend of Gram positive bacteria being more resistant to CAP treatment. However, when grown in co-culture, Gram negative P. aeruginosa was more resistant to CAP overall than as a mono-species biofilm. Emission spectra indicated OH and O, capable of structural cell wall bond breakage, were present in the plasma. This study indicates that cell wall thickness correlates with CAP inactivation times of bacteria, but cell membranes and biofilm matrix are also likely to play a role.
登录
查看更多内容
影响因子：
3.7
作者：
Conibear TC;Collins SL;Webb JS
通讯作者：
Webb JS
影响因子：
2.2
作者：
Chen, CY;Nace, GW;Irwin, PL
通讯作者：
Irwin, PL
影响因子：
2.7
作者：
Allison, DG
通讯作者：
Allison, DG
影响因子：
3.7
作者：
Delben JA;Zago CE;Tyhovych N;Duarte S;Vergani CE
通讯作者：
Vergani CE
DOI：
10.1016/j.ijantimicag.2015.02.026
发表时间：
2015-07-01
影响因子：
10.8
作者：
Flynn, Padrig B.;Higginbotham, Sarah;Gilmore, Brendan F.
通讯作者：
Gilmore, Brendan F.