Comparative transcriptome analysis of the accessory sex gland and testis from the Chinese mitten crab (Eriocheir sinensis).
Comparative transcriptome analysis of the accessory sex gland and testis from the Chinese mitten crab (Eriocheir sinensis).
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中华绒螯蟹（Eriocheir sinensis）副性腺和睾丸的比较转录组分析
DOI：
10.1371/journal.pone.0053915
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Wang Q
中科院分区：
文献类型：
--
作者：
He L;Jiang H;Cao D;Liu L;Hu S;Wang Q
副性腺（ASG）是男性生殖系统的重要组成部分，在男性生殖过程中起着提高精子生育能力的作用。已知ASG分泌的某些蛋白质与精子膜结合并影响其功能。ASG基因在中华绒螯蟹（Eriocheir sinensis）中的表达谱尚未得到广泛的研究，对该物种的遗传研究也很有限。高通量测序技术的出现使基因组资源能够在短时间内以最低的成本产生。在本研究中，我们使用Illumina测序技术进行了从头转录组测序，以建立中华鄂蚌ASG的综合转录数据集。该分析共产生33,221,284个测序读数，包括2.6 Gb的总核苷酸。Reads被组装成85,913个contigs（平均218 bp）或58,567个scaffold序列（平均292 bp），鉴定出37,955个unigenes（平均385 bp）。我们收集了所有的单基因，并将它们与已发表的中华按蚊睾丸转录组进行了比较。为了确定哪些基因可能参与了ASG的功能，因为它与精子的修饰有关，我们比较了中华中华鄂貂的ASG和睾丸转录组。我们的分析确定了在两种组织中表达水平较高和较低的特定基因，并分析了这些基因的功能，以阐明它们在精子成熟过程中的潜在作用。获取中华中华精虫ASG和睾丸的详细转录组数据有助于我们了解精子保存、转运、成熟和获能以及顶体活化的分子机制。
The accessory sex gland (ASG) is an important component of the male reproductive system, which functions to enhance the fertility of spermatozoa during male reproduction. Certain proteins secreted by the ASG are known to bind to the spermatozoa membrane and affect its function. The ASG gene expression profile in Chinese mitten crab (Eriocheir sinensis) has not been extensively studied, and limited genetic research has been conducted on this species. The advent of high-throughput sequencing technologies enables the generation of genomic resources within a short period of time and at minimal cost. In the present study, we performed de novo transcriptome sequencing to produce a comprehensive transcript dataset for the ASG of E. sinensis using Illumina sequencing technology. This analysis yielded a total of 33,221,284 sequencing reads, including 2.6 Gb of total nucleotides. Reads were assembled into 85,913 contigs (average 218 bp), or 58,567 scaffold sequences (average 292 bp), that identified 37,955 unigenes (average 385 bp). We assembled all unigenes and compared them with the published testis transcriptome from E. sinensis. In order to identify which genes may be involved in ASG function, as it pertains to modification of spermatozoa, we compared the ASG and testis transcriptome of E. sinensis. Our analysis identified specific genes with both higher and lower tissue expression levels in the two tissues, and the functions of these genes were analyzed to elucidate their potential roles during maturation of spermatozoa. Availability of detailed transcriptome data from ASG and testis in E. sinensis can assist our understanding of the molecular mechanisms involved with spermatozoa conservation, transport, maturation and capacitation and potentially acrosome activation.
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影响因子：
2.2
作者：
Gadella, BM;van Gestel, RA
通讯作者：
van Gestel, RA
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