Nutrient availability shapes the microbial community structure in sugarcane bagasse compost-derived consortia.
Nutrient availability shapes the microbial community structure in sugarcane bagasse compost-derived consortia.
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DOI：
10.1038/srep38781
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发表时间：
2016-12-12
影响因子：
4.6
通讯作者：
Polikarpov I
中科院分区：
文献类型：
--
作者：
Mello BL;Alessi AM;McQueen-Mason S;Bruce NC;Polikarpov I
微生物群落(MC)在自然生境中形成复杂的代谢网络，并通过群落结构的变化来响应环境变化。虽然MC的成员通常不适合在纯培养中培养，但当微生物作为混合培养物培养时，在实验室环境中有可能获得更多物种的多样性。为了模拟环境条件，必须使用合适的生长介质。在这里，我们检验了一种假设，即在营养有限的条件下可以维持更多的微生物多样性。利用16 S rRNA扩增元基因组学方法，对堆肥来源MC的结构进行了研究。在五周的时间里，以甘蔗渣为唯一碳源的微量培养基中的MC显示出更多的木质纤维素降解菌的多样性和丰富性。相比之下，在添加SCB的营养丰富的培养基中培养的MC具有较低的微生物多样性和有限的木质纤维分解种类。我们的方法提供了证据，证明诸如养分可获得性等因素对MC中微生物的生物多样性具有显著的选择压力。因此，营养有限的培养基可能取代细菌的通用性物种，从而为挖掘生物技术应用的新型酶提供丰富的来源。
Microbial communities (MCs) create complex metabolic networks in natural habitats and respond to environmental changes by shifts in the community structure. Although members of MCs are often not amenable for cultivation in pure culture, it is possible to obtain a greater diversity of species in the laboratory setting when microorganisms are grown as mixed cultures. In order to mimic the environmental conditions, an appropriate growth medium must be applied. Here, we examined the hypothesis that a greater diversity of microorganisms can be sustained under nutrient-limited conditions. Using a 16 S rRNA amplicon metagenomic approach, we explored the structure of a compost-derived MC. During a five-week time course the MC grown in minimal medium with sugarcane bagasse (SCB) as a sole carbon source showed greater diversity and enrichment in lignocellulose-degrading microorganisms. In contrast, a MC grown in nutrient rich medium with addition of SCB had a lower microbial diversity and limited number of lignocellulolytic species. Our approach provides evidence that factors such as nutrient availability has a significant selective pressure on the biodiversity of microorganisms in MCs. Consequently, nutrient-limited medium may displace bacterial generalist species, leading to an enriched source for mining novel enzymes for biotechnology applications.
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影响因子：
2.2
作者：
Fathallh Eida M;Nagaoka T;Wasaki J;Kouno K
通讯作者：
Kouno K
DOI：
10.1099/00207713-50-3-993
发表时间：
2000-05-01
影响因子：
2.8
作者：
Elberson, MA;Malekzadeh, F;Sowers, KR
通讯作者：
Sowers, KR
影响因子：
4
作者：
Ishii, K;Takii, S
通讯作者：
Takii, S
DOI：
10.1038/nrmicro2259
发表时间：
2010-01
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
通讯作者：
--
影响因子：
4.4
作者：
Lv, Zhiwei;Yang, Jinshui;Yuan, Hongli
通讯作者：
Yuan, Hongli