Differential susceptibility of SARS-CoV-2 in animals: Evidence of ACE2 host receptor distribution in companion animals, livestock and wildlife by immunohistochemical characterisation.
Differential susceptibility of SARS-CoV-2 in animals: Evidence of ACE2 host receptor distribution in companion animals, livestock and wildlife by immunohistochemical characterisation.
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DOI：
10.1111/tbed.14232
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发表时间：
2022-07
影响因子：
4.3
通讯作者：
Brookes SM
中科院分区：
文献类型：
--
作者：
Lean FZX;Núñez A;Spiro S;Priestnall SL;Vreman S;Bailey D;James J;Wrigglesworth E;Suarez-Bonnet A;Conceicao C;Thakur N;Byrne AMP;Ackroyd S;Delahay RJ;van der Poel WHM;Brown IH;Fooks AR;Brookes SM
血管紧张素转换酶2（ACE 2）是一种宿主细胞膜蛋白（受体），介导冠状病毒的结合，特别是呼吸道和胃肠道中的SARS冠状病毒。虽然SARS-CoV-2感染主要局限于人类，但也有许多对家畜和圈养动物的回溢（反向人畜共患病）事件。缺乏ACE 2在动物组织中的空间分布信息限制了我们对宿主物种易感性的理解。在这里，我们描述的分布ACE 2使用免疫组织化学（IHC）的组织切片来自食肉动物，有蹄类动物，灵长类动物和翼手目。水貂（Neovison vison）和雪貂（Mustela putorius furo）呼吸道的比较显示出实质性差异，表明ACE 2存在于水貂的下呼吸道，但不存在于雪貂。在某些种属中，呼吸道中ACE 2的存在受到更多限制，如猫（Felis卡图斯）的鼻甲、气管和肺中的有限免疫标记以及金黄色叙利亚仓鼠（Mesocricetus auratus）中仅鼻甲中的有限免疫标记所示。在其他物种的肺中，ACE 2可在绵羊（Ovis aries）、牛（Bos taurus）、欧洲獾（Meles meles）、猎豹（Acinonyx jubatus）、虎和狮子（Panthera spp.）的细支气管上皮上检测到。此外，ACE 2存在于鼻粘膜上皮的蝙蝠（Eptesicus cavinus），但不存在于猪（Sus scrofa arctica），牛或羊。在肠中，ACE 2免疫标记见于各种分类群的肠细胞的微绒毛（肠表面）上。这些结果提供了ACE 2在许多种属中表达的解剖学证据，这将有助于进一步了解ACE 2受体介导的病毒感染的宿主易感性和组织嗜性。
Angiotensin converting enzyme 2 (ACE2) is a host cell membrane protein (receptor) that mediates the binding of coronavirus, most notably SARS coronaviruses in the respiratory and gastrointestinal tracts. Although SARS‐CoV‐2 infection is mainly confined to humans, there have been numerous incidents of spillback (reverse zoonoses) to domestic and captive animals. An absence of information on the spatial distribution of ACE2 in animal tissues limits our understanding of host species susceptibility. Here, we describe the distribution of ACE2 using immunohistochemistry (IHC) on histological sections derived from carnivores, ungulates, primates and chiroptera. Comparison of mink (Neovison vison) and ferret (Mustela putorius furo) respiratory tracts showed substantial differences, demonstrating that ACE2 is present in the lower respiratory tract of mink but not ferrets. The presence of ACE2 in the respiratory tract in some species was much more restricted as indicated by limited immunolabelling in the nasal turbinate, trachea and lungs of cats (Felis catus) and only the nasal turbinate in the golden Syrian hamster (Mesocricetus auratus). In the lungs of other species, ACE2 could be detected on the bronchiolar epithelium of the sheep (Ovis aries), cattle (Bos taurus), European badger (Meles meles), cheetah (Acinonyx jubatus), tiger and lion (Panthera spp.). In addition, ACE2 was present in the nasal mucosa epithelium of the serotine bat (Eptesicus serotinus) but not in pig (Sus scrofa domestica), cattle or sheep. In the intestine, ACE2 immunolabelling was seen on the microvillus of enterocytes (surface of intestine) across various taxa. These results provide anatomical evidence of ACE2 expression in a number of species which will enable further understanding of host susceptibility and tissue tropism of ACE2 receptor‐mediated viral infection.
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