Assessment of proliferation, migration and differentiation potentials of bone marrow mesenchymal stem cells labeling with silica-coated and amine-modified superparamagnetic iron oxide nanoparticles
Assessment of proliferation, migration and differentiation potentials of bone marrow mesenchymal stem cells labeling with silica-coated and amine-modified superparamagnetic iron oxide nanoparticles
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二氧化硅包被和胺修饰的超顺磁性氧化铁纳米颗粒标记的骨髓间充质干细胞的增殖、迁移和分化潜能评估
DOI：
10.1007/s10616-020-00397-5
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发表时间：
2020-05
期刊：
影响因子：
2.2
通讯作者：
Mo B. W.
中科院分区：
文献类型：
--
作者：
Yao D;Liu N. N;Mo B. W.
超顺磁性氧化铁纳米粒子在临床前和临床研究中被广泛应用于细胞标记，为提高标记效率，人们开发了粒子偶联和表面修饰技术，但一些修饰后的SPION对细胞的生理活性有副作用，不能作为理想的细胞示踪剂。本研究采用氨基修饰的二氧化硅包裹的SPIO（SPIO@SiO2-NH 2，SPIO@S-N）纳米颗粒标记骨髓间充质干细胞（BM-MSCs），并对其干细胞潜能进行评价。结果表明，SPIO@S-N纳米粒子可有效标记BM-MSCs。标记后的BM-MSCs活性保持良好，迁移能力增强，但成骨和成脂能力未受影响。在激素相关性骨坏死（SAON）骨缺损模型中，将SPIO@S-N标记的BM-MSCs植入骨髓腔内，对照组可见SPIO阳性细胞归巢于缺损区周围，而PLGA/TCP支架植入组可见SPIO阳性细胞募集至缺损区。总之，SPIO@S-N纳米粒子在保持BM-MSCs增殖和分化能力的同时促进了BM-MSCs的迁移，这意味着这种纳米粒子不仅可以作为一种理想的追踪标记物，而且可以作为组织修复过程中干细胞归巢的加速器。
Superparamagnetic iron oxide nanoparticles have been widely used for cell labeling in preclinical and clinical studies, to improve labeling efficiency, particle conjugation and surface modifications are developed, but some modified SPIONs exert side-effect on physiological activity of cells, which cannot be served as ideal cell tracker. In this study, amine-modified silica-coated SPIO (SPIO@SiO2-NH2, SPIO@S-N) nanoparticles were used to label bone marrow derived mesenchymal stem cells (BM-MSCs), then the stem cell potentials were evaluated. It was found BM-MSCs could be efficiently labeled by SPIO@S-N nanoparticles. After labeling, the BM-MSCs viability kept well and the migration ability increased, but the osteogenesis and adipogenesis potentials were not impaired. In steroid associated osteonecrosis (SAON) bone defect model, stem cell implantation was performed by injection of SPIO@S-N labeled BM-MSCs into marrow cavity locally, it was found the SPIO positive cells homed to the periphery of defect region in control group, but were recruited to the defect region in poly lactic-coglycolic acid/tricalcium phosphate (PLGA/TCP) scaffold implantation group. In conclusion, SPIO@S-N nanoparticles promoted migration while retained proliferation and differentiation ability of BM-MSCs, implying this kind of nanoparticles could be served not only an ideal tracking marker but also an accelerator for stem cell homing during tissue repair.
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影响因子：
8
作者：
Kalber TL;Ordidge KL;Southern P;Loebinger MR;Kyrtatos PG;Pankhurst QA;Lythgoe MF;Janes SM
通讯作者：
Janes SM
DOI：
10.1007/978-1-61779-953-2_18
发表时间：
2012
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Jasmin;Torres, Ana Luiza Machado;Jelicks, Linda;de Carvalho, Antonio Carlos Campos;Spray, David C;Mendez-Otero, Rosalia
通讯作者：
Mendez-Otero, Rosalia
影响因子：
14
作者：
Wang, Qiwei;Chen, Bo;Gu, Ning
通讯作者：
Gu, Ning
影响因子：
0.1
作者：
Cheng Wang;Yu Wang;H. Meng;Xue-ling Yuan;Xiao-long Xu;Ai-yuan Wang;Q. Guo;Jiang Peng;Shibi Lu
通讯作者：
Cheng Wang;Yu Wang;H. Meng;Xue-ling Yuan;Xiao-long Xu;Ai-yuan Wang;Q. Guo;Jiang Peng;Shibi Lu
影响因子：
3.8
作者：
Chen, Ying-Chun;Hsiao, Jong-Kai;Huang, Dong-Ming
通讯作者：
Huang, Dong-Ming