All hematopoietic cells develop from hematopoietic stem cells through Flk2/Flt3-positive progenitor cells.
All hematopoietic cells develop from hematopoietic stem cells through Flk2/Flt3-positive progenitor cells.
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DOI：
10.1016/j.stem.2011.04.021
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发表时间：
2011-07-08
期刊：
影响因子：
23.9
通讯作者：
Forsberg, E. Camilla
中科院分区：
文献类型：
--
作者：
Boyer, Scott W.;Schroeder, Aaron V.;Smith-Berdan, Stephanie;Forsberg, E. Camilla
虽然很明显单个造血干细胞 (HSC) 能够产生所有其他造血细胞类型，但 HSC 之外的分化途径仍然存在争议。关于祖群体谱系潜力的相互矛盾的报告质疑祖群体对多谱系分化的生理贡献。在这里，我们建立了谱系追踪小鼠模型，可以直接评估体内分化途径。我们提供明确的证据表明，分化为所有造血谱系（包括巨核细胞/红系细胞类型）涉及表达 Flk2 的非自我更新祖细胞。 Flk2 阳性阶段用于稳态造血期间、辐射诱导应激后和 HSC 移植后。相反，HSC 起源和维持不包括 Flk2 阳性阶段。这些数据表明 HSC 的规范和维持不依赖于 Flk2，并且造血谱系分离发生在 Flk2 上调的下游。
While it is clear that a single hematopoietic stem cell (HSC) is capable of giving rise to all other hematopoietic cell types, the differentiation paths beyond HSC remain controversial. Contradictory reports on the lineage potential of progenitor populations have questioned the physiological contribution of progenitor populations to multilineage differentiation. Here, we established a lineage tracing mouse model that enabled direct assessment of differentiation pathways in vivo. We provide definitive evidence that differentiation into all hematopoietic lineages, including megakaryocyte/erythroid cell types, involves Flk2-expressing non-self-renewing progenitors. A Flk2-positive stage was used during steady-state hematopoiesis, after irradiation-induced stress and upon HSC transplantation. In contrast, HSC origin and maintenance do not include a Flk2-positive stage. These data demonstrate that HSC specification and maintenance are Flk2-independent, and that hematopoietic lineage separation occurs downstream of Flk2 upregulation.
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DOI：
10.1084/jem.20030107
发表时间：
2003-07-21
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
D'Amico A;Wu L
通讯作者：
Wu L
DOI：
10.1084/jem.20072168
发表时间：
2008-05-12
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Benz C;Martins VC;Radtke F;Bleul CC
通讯作者：
Bleul CC
影响因子：
64.5
作者：
Kiel, MJ;Yilmaz, ÖH;Morrison, SJ
通讯作者：
Morrison, SJ
影响因子：
64.5
作者：
Adolfsson, J;Månsson, R;Jacobsen, SEW
通讯作者：
Jacobsen, SEW
影响因子：
100.3
作者：
Ceredig, Rhodri;Rolink, Antonius G.;Brown, Geoffrey
通讯作者：
Brown, Geoffrey