Enzymatically cross-linked alginic-hyaluronic acid composite hydrogels as cell delivery vehicles.
Enzymatically cross-linked alginic-hyaluronic acid composite hydrogels as cell delivery vehicles.
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DOI：
10.1016/j.ijbiomac.2012.12.045
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发表时间：
2013-04
影响因子：
8.2
通讯作者：
Nair LS
中科院分区：
文献类型：
--
作者：
Ganesh N;Hanna C;Nair SV;Nair LS
以海藻酸钠和透明质酸为原料，制备了一种可注射的复合凝胶。在辣根过氧化物酶（HRP）和过氧化氢（H2 O2）存在下，通过酪胺修饰的海藻酸钠和透明质酸钠的氧化偶联制备酶交联的可注射凝胶。将两种三胺化聚合物溶液在10 U HRP中混合，并用1.0%H2O2处理，制备复合凝胶。透明质酸的加入对海藻酸钠凝胶的性能有显著影响。复合凝胶的百分比吸水率和储能模量被发现低于海藻酸盐凝胶。在不存在透明质酸酶的情况下，与透明质酸盐凝胶相比，藻酸盐和复合凝胶显示出较低的蛋白质释放。即使透明质酸盐凝胶在磷酸盐缓冲溶液中孵育14天后也仅显示约10%的蛋白质释放。封装在可注射凝胶中的ATDC-5细胞显示出高细胞活力。与透明质酸和海藻酸凝胶中的细胞相比，复合凝胶显示存在具有显著更高代谢活性的扩大的球形细胞。结果表明，潜在的复合材料的方法来开发共价交联的水凝胶与可调的物理，机械和生物性能。
An injectable composite gel was developed from alginic and hyaluronic acid. The ezymatically cross-linked injectable gels were prepared via the oxidative coupling of tyramine modified sodium algiante and sodium hyaluronate in the presence of horse radish peroxidase (HRP) and hydrogen peroxide (H2O2). The composite gels were prepared by mixing equal parts of the two tryaminated polymer solutions in 10U HRP and treating with 1.0% H2O2. The properties of the alginate gels were significanly affected by the addition of hyaluronic acid. The percentage water absorption and storage modulus of the composite gels were found to be lower than the alginate gels. The alginate and composite gels showed lower protein release compared to hyaluronate gels in the absence of hyaluronidase. Even hyaluronate gels showed only approximately 10% protein release after 14 days incubation in phosphate buffer solution. ATDC-5 cells encapsulated in the injectable gels showed high cell viability. The composite gels showed the presence of enlarged spherical cells with significantly higher metabolic activity compared to cells in hyaluronic and alginic acid gels. The results suggest the potential of the composite approach to develop covalently cross-linked hydrogels with tuneable physical, mechanical, and biological properties.
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影响因子：
14
作者：
Bian, Liming;Zhai, David Y.;Tous, Elena;Rai, Reena;Mauck, Robert L.;Burdick, Jason A.
通讯作者：
Burdick, Jason A.
影响因子：
14
作者：
Kim, Iris L.;Mauck, Robert L.;Burdick, Jason A.
通讯作者：
Burdick, Jason A.
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14
作者：
Banerjee, Akhilesh;Arha, Manish;Choudhary, Soumitra;Ashton, Randolph S.;Bhatia, Surita R.;Schaffer, David V.;Kane, Ravi S.
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Kane, Ravi S.
影响因子：
4
作者：
Amini, Ashley A.;Nair, Lakshmi S.
通讯作者：
Nair, Lakshmi S.
影响因子：
2.9
作者：
GUO, JF;JOURDIAN, GW;MACCALLUM, DK
通讯作者：
MACCALLUM, DK