Populus euphratica JRL Mediates ABA Response, Ionic and ROS Homeostasis in Arabidopsis under Salt Stress
Populus euphratica JRL Mediates ABA Response, Ionic and ROS Homeostasis in Arabidopsis under Salt Stress
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胡杨 JRL 介导盐胁迫下拟南芥的 ABA 反应、离子和 ROS 稳态
DOI:
10.3390/ijms20040815
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发表时间:
2019-02
影响因子:
5.6
通讯作者:
Chen Shaoliang
中科院分区:
文献类型:
--
作者:
Zhang Huilong;Deng Chen;Yao Jun;Zhang Yan-Li;Zhang Yi-Nan;Deng Shurong;Zhao Nan;Sa Gang;Zhou Xiaoyang;Lu Cunfu;Lin Shanzhi;Zhao Rui;Chen Shaoliang
Sodium chloride (NaCl) induced expression of a jacalin-related mannose-binding lectin (JRL) gene in leaves, roots, and callus cultures of Populus euphratica (salt-resistant poplar). To explore the mechanism of the PeJRL in salinity tolerance, the full length of PeJRL was cloned from P. euphratica and was transformed into Arabidopsis. PeJRL was localized to the cytoplasm in mesophyll cells. Overexpression of PeJRL in Arabidopsis significantly improved the salt tolerance of transgenic plants, in terms of seed germination, root growth, and electrolyte leakage during seedling establishment. Under NaCl stress, transgenic plants retained K+ and limited the accumulation of Na+. PeJRL-transgenic lines increased Na+ extrusion, which was associated with the upward regulation of SOS1, AHA1, and AHA2 genes encoding plasma membrane Na+/proton (H+) antiporter and H+-pumps. The activated H+-ATPases in PeJRL-overexpressed plants restricted the channel-mediated loss of K+ that was activated by NaCl-induced depolarization. Under salt stress, PeJRL–transgenic Arabidopsis maintained reactive oxygen species (ROS) homeostasis by activating the antioxidant enzymes and reducing the production of O2− through downregulation of NADPH oxidases. Of note, the PeJRL-transgenic Arabidopsis repressed abscisic acid (ABA) biosynthesis, thus reducing the ABA-elicited ROS production and the oxidative damage during the period of salt stress. A schematic model was proposed to show the mediation of PeJRL on ABA response, and ionic and ROS homeostasis under NaCl stress.
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影响因子:
3.7
作者:
Sun J;Zhang X;Deng S;Zhang C;Wang M;Ding M;Zhao R;Shen X;Zhou X;Lu C;Chen S
通讯作者:
Chen S
影响因子:
2.3
作者:
邓林;EBERHARD FRITZ;ALOYS HÜTTERMANN;王瑞刚;陈少良;ANDREA POLLE
通讯作者:
ANDREA POLLE
影响因子:
6.9
作者:
Han Y;Wang W;Sun J;Ding M;Zhao R;Deng S;Wang F;Hu Y;Wang Y;Lu Y;Du L;Hu Z;Diekmann H;Shen X;Polle A;Chen S
通讯作者:
Chen S
影响因子:
6.9
作者:
Xiang Y;Song M;Wei Z;Tong J;Zhang L;Xiao L;Ma Z;Wang Y
通讯作者:
Wang Y
影响因子:
7.4
作者:
STEWART, RRC;BEWLEY, JD
通讯作者:
BEWLEY, JD