Populus euphratica XTH overexpression enhances salinity tolerance by the development of leaf succulence in transgenic tobacco plants.
Populus euphratica XTH overexpression enhances salinity tolerance by the development of leaf succulence in transgenic tobacco plants.
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胡杨 XTH 过表达通过转基因烟草植物叶片肉质的发育增强耐盐性
DOI：
10.1093/jxb/ert229
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发表时间：
2013-11
影响因子：
6.9
通讯作者：
Chen S
中科院分区：
文献类型：
--
作者：
Han Y;Wang W;Sun J;Ding M;Zhao R;Deng S;Wang F;Hu Y;Wang Y;Lu Y;Du L;Hu Z;Diekmann H;Shen X;Polle A;Chen S
胡杨是一种耐盐树种，在长时间的盐胁迫后，其叶片会出现肉质化。在本研究中，一个假定的木葡聚糖内切转葡糖基酶/水解酶基因（PeXTH）从胡杨分离和转移到烟草植物。PeXTH仅定位于内质网和细胞壁。在根和叶的生长和存活方面，过表达PeXTH的植物比野生型烟草更耐盐。耐盐能力的增加主要是由于PeXTH过表达引起的解剖和生理变化。与野生型相比，PeXTH转基因植株的单位面积含水量高出36%，鲜干重比高出39%，这是叶片多汁的标志。然而，在PeXTH转基因植物的叶片中增加的水储存并不伴随着更大的叶片厚度，而是由于高度包装的栅栏薄壁细胞和叶肉细胞之间的细胞间空气空间较少。除了响应NaCl的盐稀释效应外，这些解剖学变化增加了叶片的保水能力，从而降低了肉质组织和叶肉细胞中盐浓度的增加。此外，叶肉细胞数量的增加减少了细胞间的空气空间，这提高了碳经济性，并导致在对照和盐处理（100- 150 mM NaCl）下的净光合作用增加47-78%。综上所述，结果表明PeXTH过表达通过在烟草植物中发育肉质叶而不肿胀来增强耐盐性。
Populus euphratica is a salt-tolerant tree species that develops leaf succulence after a prolonged period of salinity stress. In the present study, a putative xyloglucan endotransglucosylase/hydrolase gene (PeXTH) from P. euphratica was isolated and transferred to tobacco plants. PeXTH localized exclusively to the endoplasmic reticulum and cell wall. Plants overexpressing PeXTH were more salt tolerant than wild-type tobacco with respect to root and leaf growth, and survival. The increased capacity for salt tolerance was due mainly to the anatomical and physiological alterations caused by PeXTH overexpression. Compared with the wild type, PeXTH-transgenic plants contained 36% higher water content per unit area and 39% higher ratio of fresh weight to dry weight, a hallmark of leaf succulence. However, the increased water storage in the leaves in PeXTH-transgenic plants was not accompanied by greater leaf thickness but was due to highly packed palisade parenchyma cells and fewer intercellular air spaces between mesophyll cells. In addition to the salt dilution effect in response to NaCl, these anatomical changes increased leaf water-retaining capacity, which lowered the increase of salt concentration in the succulent tissues and mesophyll cells. Moreover, the increased number of mesophyll cells reduced the intercellular air space, which improved carbon economy and resulted in a 47–78% greater net photosynthesis under control and salt treatments (100–150mM NaCl). Taken together, the results indicate that PeXTH overexpression enhanced salt tolerance by the development of succulent leaves in tobacco plants without swelling.
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影响因子：
4.1
作者：
FRY, SC;SMITH, RC;MATTHEWS, KJ
通讯作者：
MATTHEWS, KJ
影响因子：
4
作者：
Gu, RS;Fonseca, S;Pais, MS
通讯作者：
Pais, MS
影响因子：
4.4
作者：
Liu, Yong;Liu, Dongcheng;Zhang, Aimin
通讯作者：
Zhang, Aimin
DOI：
10.1007/978-90-481-9370-7_8
发表时间：
2010-01-01
期刊：
PLANT ADAPTATION AND PHYTOREMEDIATION
影响因子：
--
作者：
Hameed, Mansoor;Ashraf, Muhammad;Naz, Nargis
通讯作者：
Naz, Nargis
盐诱导耐盐和盐敏感杨树叶片中 Na/K 和 ROS 稳态相关基因的表达
影响因子：
5.1
作者：
Ding, Mingquan;Hou, Peichen;Chen, Shaoliang
通讯作者：
Chen, Shaoliang