Development of the Stages of the Cycle in Mouse Seminiferous Epithelium after Transplantation of Green Fluorescent Protein-Labeled Spermatogonial Stem Cells
Development of the Stages of the Cycle in Mouse Seminiferous Epithelium after Transplantation of Green Fluorescent Protein-Labeled Spermatogonial Stem Cells
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绿色荧光蛋白标记的精原干细胞移植后小鼠生精上皮周期阶段的发展
DOI：
10.1095/biolreprod66.5.1422
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Y. Nishimune
中科院分区：
文献类型：
--
作者：
S. Ventelä;H. Ohta;M. Parvinen;Y. Nishimune
摘要 为了研究雄性生殖细胞分化的机制，将携带绿色荧光蛋白（GFP）作为转基因标记的睾丸生殖细胞移植到不育小鼠睾丸中。分离并测量了用 GFP 标记的供体生殖细胞定植的荧光阳性生精小管片段，并分析了活体压碎制剂中分化的生殖细胞。还研究了生精上皮周期正常阶段的细胞关联并用作参考。移植后两个月，集落的平均长度为1.3毫米。移植集落的细胞关联与周期正常阶段的细胞关联一致。然而，不同菌落的周期阶段不一定相同。移植后三个月，移植集落的平均长度为3.4毫米，集落各部分的细胞关联与周期相应阶段相似。即使在通过移植较高浓度的雄性生殖细胞而形成的长融合集落中，单个集落的各个区域中的细胞关联模式也相似且与周期的一些正常阶段的细胞关联模式一致。移植后6个月观察集落内不同阶段的发育。这些结果表明，精原干细胞分化的开始是随机发生的，以在不同的集落中发育出周期的不同阶段。然后，每个菌落都会在很长一段时间内表现出循环的一个阶段，即使它变成一个非常大的菌落或与其他菌落融合。这些观察结果表明存在某种同步机制。然而，到了 6 个月时，生精小管中出现了周期各阶段的正常发育。
Abstract To study the mechanism of male germ cell differentiation, testicular germ cells carrying green fluorescent protein (GFP) as a transgene marker were transplanted into infertile mouse testis. Fluorescence-positive seminiferous tubule segments colonized with GFP-labeled donor germ cells were isolated and measured, and differentiated germ cells were analyzed in living squashed preparations. Cell associations in normal stages of the seminiferous epithelial cycle were also studied and used as a reference. Two months after transplantation, the average length of the colonies was 1.3 mm. The cell associations of transplanted colonies were consistent with those of normal stages of the cycle. However, stages of the cycle were not necessarily identical in different colonies. Three months after transplantation, the average length of transplanted colonies was 3.4 mm, and the cell association in every portion of a colony was similar to that of the corresponding stage of the cycle. Even in long fused colonies made by transplantation of a higher concentration of male germ cells, the cell association patterns in various regions of a single colony were similar and consistent with those of some of the normal stages of the cycle. Development of different stages inside the colony was observed by 6 mo after transplantation. These results indicate that the commencement of spermatogonial stem cell differentiation occurs randomly to develop different stages of the cycle in different colonies. Then, each colony shows one single stage of the cycle for a long time, even if it becomes a very large colony or fuses with other colonies. These observations indicate the existence of some kind of synchronization mechanism. By 6 mo, however, normal development of the stages of the cycle appeared in seminiferous tubules.
影响因子：
--
作者：
Lonnie D. Russell;L. R. França;R. Brinster
通讯作者：
Lonnie D. Russell;L. R. França;R. Brinster
DOI：
10.1387/ijdb.9074943
发表时间：
2003-02
期刊：
The International journal of developmental biology
影响因子：
--
作者：
T. Ogawa;J. Aréchaga;M. Avarbock;R. Brinster
通讯作者：
T. Ogawa;J. Aréchaga;M. Avarbock;R. Brinster
影响因子：
3.6
作者：
Nagano, M;Avarbock, MR;Brinster, RL
通讯作者：
Brinster, RL