Silkworm expression system as a platform technology in life science.
Silkworm expression system as a platform technology in life science.
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DOI：
10.1007/s00253-009-2267-2
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发表时间：
2010-01
影响因子：
5
通讯作者：
Park, Enoch Y.
中科院分区：
文献类型：
--
作者：
Kato, Tatsuya;Kajikawa, Mizuho;Maenaka, Katsumi;Park, Enoch Y.
许多重组蛋白已在家蚕幼虫或蛹中成功表达，并用于学术和工业目的。由蚕产生的几种重组蛋白已经商业化。然而，含有目的基因的重组杆状病毒的构建需要繁琐和麻烦的步骤，并且需要长时间（3-6个月）。最近开发的杆粒，大肠杆菌和家蚕穿梭载体，消除了传统的冗长程序所需的鉴定和分离重组病毒。几项技术改进，包括半胱氨酸蛋白酶或几丁质酶缺失杆粒和伴侣辅助表达和共表达，已导致蛋白质产量显着增加和大规模生产成本降低。在家蚕产生的N-聚糖结构中发现了末端N-乙酰葡糖胺和半乳糖残基，这与昆虫细胞产生的N-聚糖结构不同。家蚕基因组的阐明为家蚕的利用开辟了新的篇章。转基因家蚕技术提供了稳定的重组蛋白生产。杆状病毒表面展示表达是家蚕幼虫源重组亚单位疫苗的低成本途径之一。利用家蚕幼虫或茧表达药学相关蛋白质，包括细胞/病毒表面蛋白、膜蛋白和鸟嘌呤核苷酸结合蛋白（G蛋白）偶联受体，已经变得非常有吸引力。家蚕生物技术是实现蛋白质高表达水平的一种创新且简单的方法，是生命科学领域非常有前途的平台技术。与“丝绸之路”一样，我们预计从亚洲到欧洲的“Bioroad”也将通过家蚕表达系统建立起来。
Many recombinant proteins have been successfully produced in silkworm larvae or pupae and used for academic and industrial purposes. Several recombinant proteins produced by silkworms have already been commercialized. However, construction of a recombinant baculovirus containing a gene of interest requires tedious and troublesome steps and takes a long time (3–6 months). The recent development of a bacmid, Escherichia coli and Bombyx mori shuttle vector, has eliminated the conventional tedious procedures required to identify and isolate recombinant viruses. Several technical improvements, including a cysteine protease or chitinase deletion bacmid and chaperone-assisted expression and coexpression, have led to significantly increased protein yields and reduced costs for large-scale production. Terminal N-acetyl glucosamine and galactose residues were found in the N-glycan structures produced by silkworms, which are different from those generated by insect cells. Genomic elucidation of silkworm has opened a new chapter in utilization of silkworm. Transgenic silkworm technology provides a stable production of recombinant protein. Baculovirus surface display expression is one of the low-cost approaches toward silkworm larvae-derived recombinant subunit vaccines. The expression of pharmaceutically relevant proteins, including cell/viral surface proteins, membrane proteins, and guanine nucleotide-binding protein (G protein) coupled receptors, using silkworm larvae or cocoons has become very attractive. Silkworm biotechnology is an innovative and easy approach to achieve high protein expression levels and is a very promising platform technology in the field of life science. Like the “Silkroad,” we expect that the “Bioroad” from Asia to Europe will be established by the silkworm expression system.
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影响因子：
64.8
作者：
Clark, Andrew G.;Eisen, Michael B.;MacCallum, Iain
通讯作者：
MacCallum, Iain
影响因子：
3.7
作者：
Li Z;Yi Y;Yin X;Zhang Z;Liu J
通讯作者：
Liu J
DOI：
10.1016/s0167-4889(99)00090-7
发表时间：
1999-08-12
影响因子：
5.1
作者：
Ishihara, K;Satoh, I;Ohuchi, K
通讯作者：
Ohuchi, K
影响因子：
3.5
作者：
Kamita, SG;Maeda, S
通讯作者：
Maeda, S
影响因子：
3.1
作者：
Hiyoshi, Masato;Kageshima, Ayano;Park, Enoch Y.
通讯作者：
Park, Enoch Y.