The Role of Neuroglobin in Retinal Hemodynamics and Metabolism: A Real-Time Study.
The Role of Neuroglobin in Retinal Hemodynamics and Metabolism: A Real-Time Study.
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DOI：
10.1167/tvst.11.7.2
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发表时间：
2022-07-08
影响因子：
3
通讯作者：
Rajendram R
中科院分区：
文献类型：
--
作者：
Kaynezhad P;Jeffery G;Bainbridge J;Sivaprasad S;Tachtsidis I;Hay-Schmidt A;Rajendram R
在这项研究中，我们使用宽带近红外光谱，一种非侵入性的光学技术，实时研究神经红蛋白在视网膜血流动力学和代谢中的可能作用。将12只C57小鼠（7只年轻，5只年老）和7只年轻的神经球蛋白敲除小鼠（Ngb-KOs）的视网膜暴露在低功率卤素光源下，利用背向反射光计算氧合血红蛋白（HbO2）、脱氧血红蛋白（hbb）和氧化细胞色素c氧化酶（oxCCO）浓度的变化。与老龄C57小鼠和Ngb-KO模型相比，年轻C57小鼠hbb、HbO2和oxCCO相关的近红外光谱信号变化程度显著高于老龄C57小鼠（P < 0.05）和Ngb-KO模型（P < 0.001）。我们的研究结果揭示了Ngb在调节视网膜功能中的可能作用，因为在敲除小鼠模型中，Ngb在视网膜中的缺失导致与血流动力学和氧化代谢相关的信号被抑制。近红外光谱能够非侵入性地检测区分神经珠蛋白敲除小鼠模型和野生型模型视网膜的特征信号。需要进一步的工作来评估信号差异的来源，以及这些差异如何与视网膜神经节细胞、双极细胞或无突细胞中神经球蛋白的存在或缺失相关。
In this study, we used broadband near-infrared spectroscopy, a non-invasive optical technique, to investigate in real time the possible role of neuroglobin in retinal hemodynamics and metabolism. Retinae of 12 C57 mice (seven young and five old) and seven young neuroglobin knockouts (Ngb-KOs) were exposed to light from a low-power halogen source, and the back-reflected light was used to calculate changes in the concentration of oxygenated hemoglobin (HbO2), deoxygenated hemoglobin (HHb), and oxidized cytochrome c oxidase (oxCCO). The degree of change in the near-infrared spectroscopy signals associated with HHb, HbO2, and oxCCO was significantly greater in young C57 mice compared to the old C57 mice (P < 0.05) and the Ngb-KO model (P < 0.005). Our results reveal a possible role of Ngb in regulating retinal function, as its absence in the retinae of a knockout mouse model led to suppressed signals that are associated with hemodynamics and oxidative metabolism. Near-infrared spectroscopy enabled the non-invasive detection of characteristic signals that differentiate between the retina of a neuroglobin knockout mouse model and that of a wild-type model. Further work is needed to evaluate the source of the signal differences and how these differences relate to the presence or absence of neuroglobin in the ganglion, bipolar, or amacrine cells of the retina.
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影响因子：
2.9
作者：
MATCHER, SJ;ELWELL, CE;DELPY, DT
通讯作者：
DELPY, DT
DOI：
10.1016/j.bbrc.2012.07.061
发表时间：
2012-08-17
影响因子：
3.1
作者：
Hundahl, C. A.;Fahrenkrug, J.;Hannibal, J.
通讯作者：
Hannibal, J.
影响因子：
4.8
作者：
Bentmann, A;Schmidt, M;Burmester, T
通讯作者：
Burmester, T
影响因子：
3.5
作者：
DELPY, DT;COPE, M;WYATT, J
通讯作者：
WYATT, J
影响因子：
3.7
作者：
Hundahl CA;Luuk H;Ilmjärv S;Falktoft B;Raida Z;Vikesaa J;Friis-Hansen L;Hay-Schmidt A
通讯作者：
Hay-Schmidt A