The effect of disease on human cardiac protein expression profiles in paired samples from right and left ventricles.
The effect of disease on human cardiac protein expression profiles in paired samples from right and left ventricles.
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DOI：
10.1186/1559-0275-11-34
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发表时间：
2014
影响因子：
3.8
通讯作者：
Suleiman MS
中科院分区：
文献类型：
--
作者：
Littlejohns B;Heesom K;Angelini GD;Suleiman MS
心脏疾病（例如冠状动脉和瓣膜）与心室细胞重塑有关。然而，来自患有不同病理的患者的左心室和右心室的心室活检很少见，因此对于疾病引起的心脏两侧以及不同疾病之间的细胞重塑知之甚少。我们假设主动脉瓣狭窄（AVS）患者和冠状动脉疾病（CAD）患者左右心室之间的蛋白质表达谱不同，并且两种疾病之间的蛋白质表达谱也不同。从患有 CAD 或 AVS 的患者中采集左心室和右心室活检。使用同量异位串联质量标记对活检组织进行蛋白质组分析，并通过反相纳米 LC-MS/MS 进行分析。所选蛋白质的蛋白质印迹显示与蛋白质组分析有很强的相关性。同一疾病的心室之间（疾病内）和不同疾病的心室之间（疾病间）的蛋白质组学分析确定了在所有相关心室活检中检测到的 500 多种蛋白质。心室和疾病状态之间的比较集中于具有相对高倍数（±1.2倍差异）和显着差异（P<0.05）的蛋白质。左心室和右心室之间的疾病内蛋白表达差异对于 AVS 患者来说主要是结构性的，对于 CAD 来说主要是信号传导/代谢的差异。通常与肥大相关的蛋白质在 AVS 组中也有所不同，但倍数差异较小。在 9 种蛋白质中检测到 AVS 和 CAD 左心室之间的疾病间差异。然而，CAD 和 AVS 患者右心室之间的疾病间差异与 73 种蛋白质的差异相关。与其他病理学相比，在一个心室中具有显着差异的大多数蛋白质在相邻心室中也具有类似的趋势。这项工作首次证明左心室和右心室具有不同的蛋白质组，并且这种差异取决于疾病的类型。右心室的疾病间差异表达更为突出。这一发现表明，一个心室中的蛋白质变化通常与相邻心室中大量蛋白质的类似趋势相关，这表明相邻心室之间存在串扰蛋白质组重塑。
Cardiac diseases (e.g. coronary and valve) are associated with ventricular cellular remodeling. However, ventricular biopsies from left and right ventricles from patients with different pathologies are rare and thus little is known about disease-induced cellular remodeling in both sides of the heart and between different diseases. We hypothesized that the protein expression profiles between right and left ventricles of patients with aortic valve stenosis (AVS) and patients with coronary artery disease (CAD) are different and that the protein profile is different between the two diseases. Left and right ventricular biopsies were collected from patients with either CAD or AVS. The biopsies were processed for proteomic analysis using isobaric tandem mass tagging and analyzed by reverse phase nano-LC-MS/MS. Western blot for selected proteins showed strong correlation with proteomic analysis. Proteomic analysis between ventricles of the same disease (intra-disease) and between ventricles of different diseases (inter-disease) identified more than 500 proteins detected in all relevant ventricular biopsies. Comparison between ventricles and disease state was focused on proteins with relatively high fold (±1.2 fold difference) and significant (P < 0.05) differences. Intra-disease protein expression differences between left and right ventricles were largely structural for AVS patients and largely signaling/metabolism for CAD. Proteins commonly associated with hypertrophy were also different in the AVS group but with lower fold difference. Inter-disease differences between left ventricles of AVS and CAD were detected in 9 proteins. However, inter-disease differences between the right ventricles of CAD and AVS patients were associated with differences in 73 proteins. The majority of proteins which had a significant difference in one ventricle compared to the other pathology also had a similar trend in the adjacent ventricle. This work demonstrates for the first time that left and right ventricles have a different proteome and that the difference is dependent on the type of disease. Inter-disease differential expression was more prominent for right ventricles. The finding that a protein change in one ventricle was often associated with a similar trend in the adjacent ventricle for a large number of proteins suggests cross-talk proteome remodeling between adjacent ventricles.
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影响因子：
--
作者：
Abdul-Ghani S;Heesom KJ;Angelini GD;Suleiman MS
通讯作者：
Suleiman MS
DOI：
10.1155/2012/342659
发表时间：
2012
期刊：
International journal of proteomics
影响因子：
--
作者：
Bergquist J;Baykut G;Bergquist M;Witt M;Mayer FJ;Baykut D
通讯作者：
Baykut D
影响因子：
4.8
作者：
Kapoor, Neeraj;Gupta, Ruchi;Sakmar, Thomas P.
通讯作者：
Sakmar, Thomas P.
影响因子：
20.1
作者：
Barth, AS;Merk, S;Nabauer, M
通讯作者：
Nabauer, M
DOI：
10.1253/jcj.65.445
发表时间：
2001-05-01
期刊：
JAPANESE CIRCULATION JOURNAL-ENGLISH EDITION
影响因子：
--
作者：
Baba, H;Ishiwata, T;Asano, G
通讯作者：
Asano, G