Non-muscle alpha-dystroglycan is involved in epithelial development.
Non-muscle alpha-dystroglycan is involved in epithelial development.
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DOI：
10.1083/jcb.130.1.79
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发表时间：
1995-07
影响因子：
7.8
通讯作者：
EKBLOM, P
中科院分区：
文献类型：
--
作者：
DURBEEJ, M;LARSSON, E;IBRAGHIMOVBESKROVNAYA, O;ROBERDS, SL;CAMPBELL, KP;EKBLOM, P
肌营养不良蛋白聚糖复合物是肌肉中细胞骨架和基底膜之间的跨膜连接。复合物的组分之一α-肌营养不良蛋白聚糖结合肌肉的层粘连蛋白（层粘连蛋白-2）和肌肉基底膜的聚集蛋白。在非肌肉组织中也检测到了肌营养不良聚糖，但其在非肌肉组织中的生理作用仍不清楚。在这里，我们表明，肌营养不良蛋白聚糖在小鼠发育过程中的非肌肉组织中的上皮细胞中表达。原位杂交显示肌营养不良蛋白聚糖mRNA的强表达在所有研究的上皮片，但不是在内皮细胞或间充质。间充质向上皮的转化发生在肾发育期间，并且胚胎肾用于研究α-肌营养不良聚糖对于上皮分化的作用。在后肾间充质的体外培养过程中，在第二天可以看到上皮分化的第一个形态学迹象。北方印迹显示在体外发育的第二天肌营养不良蛋白聚糖mRNA明显增加。先前显示层粘连蛋白α 1链在第二天表达的类似增加。免疫荧光显示肌营养不良蛋白聚糖严格位于发育中的肾上皮细胞的基底侧。已知阻断α-肌营养不良蛋白聚糖与层粘连蛋白-1结合的单克隆抗体扰乱了肾器官培养物中上皮的发育，而对照抗体没有这样做。我们认为肌营养不良蛋白聚糖复合物在上皮形态发生过程中作为基底膜成分的受体。这很可能涉及α-肌营养不良聚糖与层粘连蛋白-1的E3片段的结合。
The dystroglycan complex is a transmembrane linkage between the cytoskeleton and the basement membrane in muscle. One of the components of the complex, alpha-dystroglycan binds both laminin of muscle (laminin-2) and agrin of muscle basement membranes. Dystroglycan has been detected in nonmuscle tissues as well, but the physiological role in nonmuscle tissues has remained unknown. Here we show that dystroglycan during mouse development in nonmuscle tissues is expressed in epithelium. In situ hybridization revealed strong expression of dystroglycan mRNA in all studied epithelial sheets, but not in endothelium or mesenchyme. Conversion of mesenchyme to epithelium occurs during kidney development, and the embryonic kidney was used to study the role of alpha-dystroglycan for epithelial differentiation. During in vitro culture of the metanephric mesenchyme, the first morphological signs of epithelial differentiation can be seen on day two. Northern blots revealed a clear increase in dystroglycan mRNA on day two of in vitro development. A similar increase of expression on day two was previously shown for laminin alpha 1 chain. Immunofluorescence showed that dystroglycan is strictly located on the basal side of developing kidney epithelial cells. Monoclonal antibodies known to block binding of alpha-dystroglycan to laminin-1 perturbed development of epithelium in kidney organ culture, whereas control antibodies did not do so. We suggest that the dystroglycan complex acts as a receptor for basement membrane components during epithelial morphogenesis. It is likely that this involves binding of alpha- dystroglycan to E3 fragment of laminin-1.
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DOI：
10.1083/jcb.122.4.809
发表时间：
1993-08
期刊：
The Journal of cell biology
影响因子：
--
作者：
Ervasti JM;Campbell KP
通讯作者：
Campbell KP
整联蛋白的α1-α6亚基特征是在发展中和成人人类肾脏的不同段中表达。
DOI：
10.1083/jcb.111.3.1245
发表时间：
1990-09
期刊：
The Journal of cell biology
影响因子：
--
作者：
Korhonen M;Ylänne J;Laitinen L;Virtanen I
通讯作者：
Virtanen I
影响因子：
64.5
作者：
CAMPANELLI, JT;ROBERDS, SL;SCHELLER, RH
通讯作者：
SCHELLER, RH
影响因子：
64.5
作者：
EKBLOM, M;KLEIN, G;EKBLOM, P
通讯作者：
EKBLOM, P
影响因子：
16.2
作者：
ERNFORS, P;WETMORE, C;PERSSON, H
通讯作者：
PERSSON, H