Circadian pacemaker neurons change synaptic contacts across the day.
Circadian pacemaker neurons change synaptic contacts across the day.
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DOI：
10.1016/j.cub.2014.07.063
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发表时间：
2014-09-22
期刊：
影响因子：
9.2
通讯作者：
Fernanda Ceriani, Maria
中科院分区：
文献类型：
--
作者：
Axel Gorostiza, E.;Depetris-Chauvin, Ana;Frenkel, Lia;Pirez, Nicolas;Fernanda Ceriani, Maria
休息和活动的每日周期是生理学昼夜节律控制的常见例子。在果蝇中，有节奏的运动周期依赖于分成七个簇的150-200个神经元的活动。来自许多实验室的工作指出小腹侧神经元（sLNvs）对于运动节律的昼夜控制是必不可少的。sLNv神经元经历其轴突投射的昼夜节律重塑，从而打开了这些相关昼夜节律起搏器的连接的昼夜节律控制的可能性。在这里，我们表明，昼夜节律可塑性的sLNv轴突预测有进一步的影响，而不仅仅是结构的变化。首先，我们发现，日常结构可塑性的程度超过了原来描述的强调，在fasciculation的程度，以及轴突终端的延伸或修剪的变化可能涉及。有趣的是，活跃区域的数量沿着而变化，这支持了一个有吸引力的假设，即在深夜和第二天早上之间，新的突触形成，而其他突触被拆除。更值得注意的是，充分利用GFP跨突触伴侣重建（GRASP）技术，我们发现，除了添加或移除新的突触之外，sLNv神经元在一天中的不同时间沿着接触不同的突触伴侣。这些结果使我们提出，昼夜节律网络，特别是sLNv神经元，通过改变信息传播的路径，协调了白天和黑夜之间的一些生理和行为差异。
Daily cycles of rest and activity are a common example of circadian control of physiology. In Drosophila rhythmic locomotor cycles rely on the activity of 150-200 neurons grouped in seven clusters. Work from many laboratories points to the small Lateral Neurons ventral (sLNvs) as essential for circadian control of locomotor rhythmicity. sLNv neurons undergo circadian remodeling of their axonal projections opening the possibility for a circadian control of connectivity of these relevant circadian pacemakers. Here we show that circadian plasticity of the sLNv axonal projections has further implications than mere structural changes. First, we found that the degree of daily structural plasticity exceeds that originally described underscoring that changes in the degree of fasciculation as well as extension or pruning of axonal terminals could be involved. Interestingly, the quantity of active zones changes along the day, lending support to the attractive hypothesis that new synapses are formed while others are dismantled between late night and the following morning. More remarkably, taking full advantage of the GFP Reconstitution Across Synaptic Partners (GRASP) technique we showed that, in addition to new synapses being added or removed, sLNv neurons contact different synaptic partners at different times along the day. These results lead us to propose that the circadian network, and in particular the sLNv neurons, orchestrates some of the physiological and behavioral differences between day and night by changing the path through which information travels.
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DOI：
10.1126/science.1202839
发表时间：
2011-06-24
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Bushey D;Tononi G;Cirelli C
通讯作者：
Cirelli C
DOI：
10.1126/science.1166673
发表时间：
2009-04-03
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Gilestro GF;Tononi G;Cirelli C
通讯作者：
Cirelli C
影响因子：
1.9
作者：
HEISENBERG, M;BORST, A;BYERS, D
通讯作者：
BYERS, D
影响因子：
2.5
作者：
Helfrich-Förster, C
通讯作者：
Helfrich-Förster, C
影响因子：
64.8
作者：
Grima, B;Chèlot, E;Rouyer, F
通讯作者：
Rouyer, F