The effect of oxyhemoglobin on the proliferation and migration of cultured vascular smooth muscle cells
The effect of oxyhemoglobin on the proliferation and migration of cultured vascular smooth muscle cells
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氧合血红蛋白对培养血管平滑肌细胞增殖和迁移的影响
DOI：
10.1007/978-3-211-75718-5_38
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
冯华
中科院分区：
文献类型：
--
作者：
刘智;陈志;JH Zhang;朱刚;唐卫华;冯华
血管平滑肌细胞（VSMC）的增殖和迁移以前被认为是血管疾病的中心特征。然而，血管细胞增殖和迁移在蛛网膜下腔出血（SAH）后脑血管痉挛中的潜在作用尚未得到广泛研究。由于氧合血红蛋白（oxyHb）与脑血管痉挛的发病机制有关，本研究采用1-200 μM的oxyHb模拟临床情况，观察了oxyHb对培养的大鼠主动脉平滑肌细胞增殖和迁移的影响。所用的形态学和生物化学技术包括甲基噻唑基四唑（MTT）测定，流式细胞术细胞周期分析，单层损伤试验，和Boyden的室迁移试验。结果表明，低浓度的oxyHb（1 μM、10 μM）可促进VSMC的增殖和迁移，而高浓度的oxyHb（100 μM、200 μM）则相反，抑制VSMC的增殖和迁移。VSMC的增殖能力在氧合血红蛋白的影响下呈双向变化。这与临床过程一致。因此，抑制VSMC增殖和迁移可能有助于SAH后脑血管痉挛的治疗。
Vascular smooth muscle cells (VSMC) proliferation and migration have previously been regarded as central features in vascular disease. However, the potential roles of vascular cell proliferation and migration in cerebral vasospasm following subarachnoid hemorrhage (SAH) have not been extensively studied. Since oxyhemoglobin (oxyHb) is implicated in the pathogenesis of cerebral vasospasm, 1–200 μM of oxyHb was used in this study to mimic the clinical situation, and the effects of oxyHb on the proliferation and migration of cultured rat aortic smooth muscle cells were investigated. The morphological and biochemical techniques used included the methyl thiazolyl tetrazolium (MTT) assay, flow cytometry cell cycle analysis, monolayer-wounding assay, and Boyden’s chamber migration assay. Results show that low concentration of oxyHb (1 μM, 10 μM) would increase the proliferation and migration of VSMC, but on the contrary, high concentration of oxyHb (100 μM, 200 μM) would inhibit the proliferation and migration of VSMC. The proliferative ability of VSMC turned out to be a bidirectional alteration under the influence of oxyHb. It was consistent with the clinical course. Therefore, anti-VSMC proliferation and migration may be useful in the therapy of cerebral vasospasm after SAH.
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DOI：
--
发表时间：
1996-11
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
T. D. Oury;Brian J. Day;J. Crapo
通讯作者：
T. D. Oury;Brian J. Day;J. Crapo
影响因子：
4.1
作者：
L. Conway;L. W. Mcdonald
通讯作者：
L. Conway;L. W. Mcdonald
DOI：
--
发表时间：
1985-03
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
--
作者：
W. Martin;G. Villani;D. Jothianandan;R. Furchgott
通讯作者：
W. Martin;G. Villani;D. Jothianandan;R. Furchgott
影响因子：
1.9
作者：
Robert R. Smith;B. Clower;J. Cruse;Yutaka Honma;Joseph L. Haining
通讯作者：
Robert R. Smith;B. Clower;J. Cruse;Yutaka Honma;Joseph L. Haining
影响因子：
2.5
作者：
H. Ohkuma;Shigeharu Suzuki;K. Ogane
通讯作者：
H. Ohkuma;Shigeharu Suzuki;K. Ogane