Screening of Rhizospheric Actinomycetes for Various In-vitro and In-vivo Plant Growth Promoting (PGP) Traits and for Agroactive Compounds.
Screening of Rhizospheric Actinomycetes for Various In-vitro and In-vivo Plant Growth Promoting (PGP) Traits and for Agroactive Compounds.
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DOI：
10.3389/fmicb.2016.01334
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发表时间：
2016
影响因子：
5.2
通讯作者：
Sajid I
中科院分区：
文献类型：
--
作者：
Anwar S;Ali B;Sajid I
本研究从巴基斯坦旁遮普省不同的小麦和番茄田中分离到98种根际放线菌。对分离物进行了形态、生化和遗传鉴定，并进行了各种植物生长促进（PGP）性状的综合体外筛选。通过16s rRNA基因测序，筛选到的PGP根瘤菌（PGPRs）与链霉菌属（Streptomyces）中不同种属的遗传相似性最高，达到98 ~ 99%。在500 μg/ml l -色氨酸条件下，产吲哚乙酸活性最高的是nobilis Streptomyces WA-3、Kunmingenesis WC-3和enissocaesilis TA-3，分别产79.5、79.23和69.26 μg/ml IAA。可溶性磷酸盐含量最高的是Streptomyces sp. WA-1 （72.13 mg/100 ml）和s.d jakartensis TB-4 （70.36 mg/100 ml）。所有分离的根杆菌对铁载体、氨和氰化氢的生产均呈阳性。菌株WD-3的acc -脱氨酶含量最高（1.9 mmol /l）。采用小麦（Triticum aestivum）种子接种方法进行体内筛选、种子萌发和植株生长试验。与经水处理的对照植株相比，水杨酸WA-3植株的茎长显著增加（65%），根长显著增加（81%）。与经水处理的对照植株相比，水处理后的白蜡蜡WA-3植株鲜重增幅最大（84%），干重增幅最大（85%）。在叶片数方面，与对照植株相比，豪门WA-3显著增加（27%），豪门WA-3显著增加（30%）根数。综上所述，这些根际PGP链霉菌是促进小麦作物生长和增产的生物肥料，可用于各种农业活性化合物的商业化生产。
In this study 98 rhizospheric actinomycetes were isolated from different wheat and tomato fields, Punjab, Pakistan. The isolates were characterized morphologically, biochemically, and genetically and were subjected to a comprehensive in vitro screening for various plant growth promoting (PGP) traits. About 30% of the isolates screened were found to be the promising PGP rhizobacteria (PGPRs), which exhibited maximum genetic similarity (up to 98–99%) with different species of the genus Streptomyces by using16S rRNA gene sequencing. The most active indole acetic acid (IAA) producer Streptomyces nobilis WA-3, Streptomyces Kunmingenesis WC-3, and Streptomyces enissocaesilis TA-3 produce 79.5, 79.23, and 69.26 μg/ml IAA respectively at 500 μg/ml L-tryptophan. The highest concentration of soluble phosphate was produced by Streptomyces sp. WA-1 (72.13 mg/100 ml) and S. djakartensis TB-4 (70.36 mg/100 ml). All rhizobacterial isolates were positive for siderophore, ammonia, and hydrogen cyanide production. Strain S. mutabilis WD-3 showed highest concentration of ACC-deaminase (1.9 mmol /l). For in-vivo screening, seed germination, and plant growth experiment were conducted by inoculating wheat (Triticum aestivum) seeds with the six selected isolates. Significant increases in shoot length was observed with S. nobilis WA-3 (65%), increased root length was recorded in case of S. nobilis WA-3 (81%) as compared to water treated control plants. Maximum increases in plant fresh weight were recorded with S. nobilis WA-3 (84%), increased plant dry weight was recorded in case of S. nobilis WA-3 (85%) as compared to water treated control plants. In case of number of leaves, significant increase was recorded with S. nobilis WA-3 (27%) and significant increase in case of number of roots were recorded in case of strain S. nobilis WA-3 (30%) as compared to control plants. Over all the study revealed that these rhizospheric PGP Streptomyces are good candidates to be developed as bioferlizers for growth promotion and yield enhancement in wheat crop and can be exploited for the commercial production of different agro-active compounds.
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影响因子：
4.1
作者：
Khamna, Sutthinan;Yokota, Akira;Lumyong, Saisamorn
通讯作者：
Lumyong, Saisamorn
影响因子：
4.1
作者：
Deepa, C. K.;Dastager, Syed G.;Pandey, Ashok
通讯作者：
Pandey, Ashok
影响因子：
4.1
作者：
Errakhi, Rafik;Bouteau, Francois;Barakate, Mustapha
通讯作者：
Barakate, Mustapha
DOI：
10.1016/j.jtemb.2005.02.006
发表时间：
2005-01-01
影响因子：
3.5
作者：
Khan, AG
通讯作者：
Khan, AG
影响因子：
4
作者：
Khalid, A;Arshad, M;Zahir, ZA
通讯作者：
Zahir, ZA