A microwell-based impedance sensor on an insertable microneedle for real-time in vivo cytokine detection.
A microwell-based impedance sensor on an insertable microneedle for real-time in vivo cytokine detection.
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DOI：
10.1038/s41378-021-00297-4
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发表时间：
2021
影响因子：
7.9
通讯作者：
Allen MG
中科院分区：
文献类型：
--
作者：
Song N;Xie P;Shen W;Oh H;Zhang Y;Vitale F;Javanmard M;Allen MG
用于即时诊断的基于阻抗的蛋白质检测传感器需要定量特异性以及快速或实时操作。此外，这些传感器的微加工可以导致适合体内操作的因子的形成。在此，我们提出了微加工的针状微孔阻抗传感器，用于快速样品响应、无标记检测细胞因子和其他生物标志物。微针形状因素允许传感器用于经皮或经血管传感应用。体外实验表征证实了传感器对多种目标蛋白的特异性和敏感性。机械特性表明微针对于经皮插入具有足够的鲁棒性，并且在插入后保留了传感器功能。我们进一步利用这些传感器对内源表达 hIL8 的转基因小鼠血液中人白细胞介素 8 (hIL8) 浓度水平进行实时体内定量。为了评估传感器功能，通过 ELISA 对来自同一小鼠的血清样本中的 hIL8 浓度水平进行了定量。在表达 hIL8 浓度从 62 pg/mL 到 539 ng/mL 的多个转基因小鼠中观察到血液中实时体内传感器读数与随后的 ELISA 血清测定之间的极好一致性。
Impedance-based protein detection sensors for point-of-care diagnostics require quantitative specificity, as well as rapid or real-time operation. Furthermore, microfabrication of these sensors can lead to the formation of factors suitable for in vivo operation. Herein, we present microfabricated needle-shaped microwell impedance sensors for rapid-sample-to-answer, label-free detection of cytokines, and other biomarkers. The microneedle form factor allows sensors to be utilized in transcutaneous or transvascular sensing applications. In vitro, experimental characterization confirmed sensor specificity and sensitivity to multiple proteins of interest. Mechanical characterization demonstrated sufficient microneedle robustness for transcutaneous insertion, as well as preserved sensor function postinsertion. We further utilized these sensors to carry out real-time in vivo quantification of human interleukin 8 (hIL8) concentration levels in the blood of transgenic mice that endogenously express hIL8. To assess sensor functionality, hIL8 concentration levels in serum samples from the same mice were quantified by ELISA. Excellent agreement between real-time in vivo sensor readings in blood and subsequent ELISA serum assays was observed over multiple transgenic mice expressing hIL8 concentrations from 62 pg/mL to 539 ng/mL.
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DOI：
10.1073/pnas.95.21.12271
发表时间：
1998-10-13
影响因子：
11.1
作者：
Höök, F;Rodahl, M;Brzezinski, P
通讯作者：
Brzezinski, P
影响因子：
28.1
作者：
Boutry, Clementine M.;Beker, Levent;Bao, Zhenan
通讯作者：
Bao, Zhenan
影响因子：
3.8
作者：
Chen, Alvin I.;Balter, Max L.;Yarmush, Martin L.
通讯作者：
Yarmush, Martin L.
影响因子：
5.4
作者：
Ramachandran, N;Larson, DN;LaBaer, J
通讯作者：
LaBaer, J
影响因子：
--
作者：
Campagnolo, C;Meyers, KJ;Batt, CA
通讯作者：
Batt, CA