GC-MS Based Metabolomics Reveals the Synergistic Mechanism of Gardeniae Fructus-Forsythiae Fructus Herb Pair in Lipopolysaccharide-Induced Acute Lung Injury Mouse Model.
GC-MS Based Metabolomics Reveals the Synergistic Mechanism of Gardeniae Fructus-Forsythiae Fructus Herb Pair in Lipopolysaccharide-Induced Acute Lung Injury Mouse Model.
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基于GC-MS的代谢组学揭示了栀子-连翘药对在脂多糖诱导的急性肺损伤小鼠模型中的协同机制
DOI：
10.1155/2021/8064557
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Zhou W
中科院分区：
文献类型：
--
作者：
Wu W;Lin H;Yin A;Shen C;Zhou H;Wang M;Yu H;Wang H;Zhang Z;Zhou W
兼容性仍然是中药的关键和重要特性之一。栀子 - 连翘药对是清热解毒方剂的一个缩影，在临床环境中被广泛用于治疗细菌性肺炎。然而，关于它们协同作用的报道很少。因此，本研究利用脂多糖（LPS）诱导的急性肺损伤（ALI）小鼠模型，通过基于气相色谱 - 质谱（GC - MS）的代谢组学研究了它们的兼容性。通过正交偏最小二乘法判别分析（OPLS - DA）模式中变量重要性投影（VIP）> 1以及P < 0.05来鉴定差异代谢物。生化和组织病理学结果表明，栀子 - 连翘药对在LPS诱导的ALI模型中比其单味药煎剂具有更有前景的肺保护作用。从代谢组学研究来看，在空白对照组与模型组中发现32种差异代谢物，在连翘组与模型组中发现21种差异代谢物，在栀子组与模型组中发现21种差异代谢物，在栀子 - 连翘药对组与模型组中发现20种差异代谢物。其中，3 - 羟基丁酸、丙氨酸、间苯二甲酸和对苯二甲酸的水平在连翘组中显著恢复，而硅烷醇和胆固醇在栀子组中显著恢复。对于栀子 - 连翘治疗，具有抗炎特性的代谢物山嵛酸的量增加，而促炎代谢物棕榈酸的量减少。同时，这两种生物标志物比栀子或连翘单独治疗时恢复得更显著，这表明连翘与栀子的协同作用可能归因于脂肪酸代谢途径的恢复。
Compatibility remains among the crucial and significant characteristics of traditional Chinese medicines. The Gardeniae Fructus (FG)-Forsythiae Fructus (FF) herb pair, an epitome of formulations for heat-clearing and detoxification, is extensively used to treat bacterial pneumonia in clinical settings. However, there are few reports on their synergistic effects. This study thus investigated their compatibility by GC-MS based metabolomics using a lipopolysaccharide (LPS)-induced acute lung injury (ALI) mouse model. Differential metabolites were identified by both variable importance in the projection (VIP) > 1 in orthogonal partial least-squares discriminant analysis (OPLS-DA) mode and P < 0.05. Results of biochemistry and histopathology indicated that FG-FF herb pair exerted more promising lung protective effect than its individual decoction against the LPS-induced ALI model. From the metabolomics study, 32 differential metabolites in vehicle vs. model groups, 21 differential metabolites in FF vs. model groups, 21 differential metabolites in FG vs. model groups, and 20 differential metabolites in FG-FF herb pair vs. model groups were found. Among them, the levels of 3-hydroxybutyric acid, alanine, isophthalic acid, and terephthalic acid were restored significantly in the FF group, while silanol and cholesterol were restored significantly in the FG group. For FG-FF treatment, the amount of behenic acid, a metabolite with anti-inflammatory properties, was increased, while palmitic acid, a proinflammatory metabolite, was decreased. Meanwhile, the two biomarkers were restored more significantly than that by FG or FF treatment, which indicated that the synergistic effects by FF coupled with FG might be attributed to restoring fatty acids metabolic pathway.
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影响因子：
1.7
作者：
Liu, Hao;Chen, Yong-Feng;Zhang, Hai-Yan
通讯作者：
Zhang, Hai-Yan
影响因子：
5.7
作者：
Bao, Jiaolin;Ding, Renbo;He, Chengwei
通讯作者：
He, Chengwei
灵芝多糖对急性肺损伤大鼠的保护作用及其代谢组学
DOI：
10.1016/j.ijbiomac.2019.10.010
发表时间：
2020-01-01
影响因子：
8.2
作者：
Li, Lu;Fu, Wang-wei;Xie, Ming-yong
通讯作者：
Xie, Ming-yong
影响因子：
25.7
作者：
Chen Y;Ouyang X;Hoque R;Garcia-Martinez I;Yousaf MN;Tonack S;Offermanns S;Dubuquoy L;Louvet A;Mathurin P;Massey V;Schnabl B;Bataller RA;Mehal WZ
通讯作者：
Mehal WZ
影响因子：
5.4
作者：
Hu, Lianqi;Wang, Yaqi;Yang, Ming
通讯作者：
Yang, Ming