Micromotion-induced strain fields influence early stages of repair at bone-implant interfaces.
Micromotion-induced strain fields influence early stages of repair at bone-implant interfaces.
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DOI：
10.1016/j.actbio.2013.01.014
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发表时间：
2013-05
影响因子：
9.7
通讯作者：
Nanci, Antonio
中科院分区：
文献类型：
--
作者：
Wazen, Rima M.;Currey, Jennifer A.;Guo, Hongqiang;Brunski, John B.;Helms, Jill A.;Nanci, Antonio
种植体载荷可以在骨-种植体界面产生微运动。种植体微动引起的界面应变可能对组织愈合反应有调节作用。过度的微动会导致纤维包封和植入物松动。我们的目的是表征界面应变对小鼠胫骨植入物周围骨再生的影响。使用微动系统在(1)种植体与骨之间没有初始接触和(2)骨与种植体直接接触的条件下产生应变。针形植入物和螺钉形植入物分别承受150 μm和300 μm的位移，60个周期/天，持续7天。在5只动物体内放置针形植入物，每天进行3次150 μm位移，每次60个周期。两种界面的对照种植体在整个愈合期间都是稳定的。实验应变分析、微层析成像、基于图像的位移映射和有限元模拟用于表征界面应变场。制备钙化组织切片，用Goldner染色评价高应变区和低应变区的组织反应。在稳定种植体中，骨形成在种植体周围一致发生。在受微运动影响的种植体中，高应变浓度区域（例如bbb30 %）的骨再生被破坏，而低应变值则允许骨形成。增加种植体位移或每天循环次数也会改变应变分布并干扰骨愈合。这些结果表明，种植体的微运动和相关的界面应变场都有助于调节骨-种植体愈合界面的力学生物学。
Implant loading can create micromotion at the bone-implant interface. The interfacial strain associated with implant micromotion could contribute to regulating the tissue healing response. Excessive micromotion can lead to fibrous encapsulation and implant loosening. Our objective was to characterize the influence of interfacial strain on bone regeneration around implants in mouse tibiae. A micromotion system was used to create strain under conditions of (1) no initial contact between implant and bone, and (2) a direct bone-implant contact. Pin- and screw-shaped implants were subjected to displacements of 150 μm or 300 μm, 60 cycles/day, for 7 days. Pin-shaped implants placed in 5 animals were subjected to 3 sessions of 150 μm displacement per day, with 60 cycles per session. Control implants in both types of interfaces were stabilized throughout the healing period. Experimental strain analyses, microtomography, image-based displacement mapping, and finite element simulations were used to characterize interfacial strain fields. Calcified tissue sections were prepared and stained with Goldner to evaluate tissue reaction in higher and lower strain regions. In stable implants, bone formation occurred consistently around the implants. In implants subjected to micromotion, bone regeneration was disrupted in areas of high strain concentrations (e.g. > 30%), whereas lower strain values were permissive of bone formation. Increasing implant displacement or number of cycles per day also changed the strain distribution and disturbed bone healing. These results indicate that not only implant micromotion but also the associated interfacial strain field contributes to regulating the interfacial mechanobiology at healing bone-implant interfaces.
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