Discovery of a novel small secreted protein family with conserved N-terminal IGY motif in Dikarya fungi.
Discovery of a novel small secreted protein family with conserved N-terminal IGY motif in Dikarya fungi.
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在 Dikarya 真菌中发现具有保守 N 末端 IGY 基序的新型小分泌蛋白家族
DOI：
10.1186/1471-2164-15-1151
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发表时间：
2014-12-20
期刊：
影响因子：
4.4
通讯作者：
Huang M
中科院分区：
文献类型：
--
作者：
Cheng Q;Wang H;Xu B;Zhu S;Hu L;Huang M
植物病原真菌利用小分泌蛋白（ssp）作为其成功定植的重要策略工具。ssp通常是种特异性的，到目前为止，只有少数广泛分布的ssp已被确定。结果在杨树真菌病原菌褐马松病中鉴定出一个新的SSP家族，共有107个成员，占其分泌组的17%以上。基于n端保守的三个氨基酸，我们将这些蛋白命名为IGY蛋白（IGYPs）。尽管IGYPs之间的序列相似性总体较低；它们显示出保守的N和c端基序和统一的基因结构。通过RT-PCR-seq，我们分析了igypgene模型，并验证了它们在感染过程中作为活性基因的表达。在其他25种Dikarya真菌中也发现了IGYP同源物，这些真菌都具有保守的基序和相同的基因结构。此外，来自11种真菌的18个IGYPs也具有相似的基因组背景。Real-time RT-PCR结果显示8mbigyps在生物营养阶段高表达。有趣的是，12个MbIGYPs的瞬时实验表明，MbIGYP13蛋白诱导了抗性杨树无性系的细胞死亡。结论共在26种Dikarya亚界真菌中发现了154个IGYPs。基因结构和基因组环境分析表明，atigypp起源于一个共同的祖先。立即通知。brunnea，高度分化的MbIGYPs的扩张可能与植物-病原体军备竞赛有关。
BackgroundSmall secreted proteins (SSPs) are employed by plant pathogenic fungi as essential strategic tools for their successful colonization. SSPs are often species-specific and so far only a few widely phylogenetically distributed SSPs have been identified.ResultsA novel fungal SSP family consisting of 107 members was identified in the poplar tree fungal pathogenMarssonina brunnea, which accounts for over 17% of its secretome. We named these proteins IGY proteins (IGYPs) based on the conserved three amino acids at the N-terminus. In spite of overall low sequence similarity among IGYPs; they showed conserved N- and C-terminal motifs and a unified gene structure. By RT-PCR-seq, we analyzed theIGYPgene models and validated their expressions as active genes during infection. IGYP homologues were also found in 25 other Dikarya fungal species, all of which shared conserved motifs and the same gene structure. Furthermore, 18 IGYPs from 11 fungi also shared similar genomic contexts. Real-time RT-PCR showed that 8MbIGYPswere highly expressed in the biotrophic stage. Interestingly, transient assay of 12 MbIGYPs showed that the MbIGYP13 protein induced cell death in resistant poplar clones.ConclusionsIn total, 154 IGYPs in 26 fungi of the Dikarya subkingdom were discovered. Gene structure and genomic context analyses indicated thatIGYPsoriginated from a common ancestor. InM. brunnea, the expansion of highly divergent MbIGYPs possibly is associated with plant-pathogen arms race.
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DOI：
10.1126/science.1171652
发表时间：
2009-05-08
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Panstruga R;Dodds PN
通讯作者：
Dodds PN
基因组和转录组分析的植物致病性真菌的生活方式过渡。
影响因子：
30.8
作者：
O'Connell, Richard J.;Thon, Michael R.;Hacquard, Stephane;Amyotte, Stefan G.;Kleemann, Jochen;Torres, Maria F.;Damm, Ulrike;Buiate, Ester A.;Epstein, Lynn;Alkan, Noam;Altmueller, Janine;Alvarado-Balderrama, Lucia;Bauser, Christopher A.;Becker, Christian;Birren, Bruce W.;Chen, Zehua;Choi, Jaeyoung;Crouch, Jo Anne;Duvick, Jonathan P.;Farman, Mark A.;Gan, Pamela;Heiman, David;Henrissat, Bernard;Howard, Richard J.;Kabbage, Mehdi;Koch, Christian;Kracher, Barbara;Kubo, Yasuyuki;Law, Audrey D.;Lebrun, Marc-Henri;Lee, Yong-Hwan;Miyara, Itay;Moore, Neil;Neumann, Ulla;Nordstroem, Karl;Panaccione, Daniel G.;Panstruga, Ralph;Place, Michael;Proctor, Robert H.;Prusky, Dov;Rech, Gabriel;Reinhardt, Richard;Rollins, Jeffrey A.;Rounsley, Steve;Schardl, Christopher L.;Schwartz, David C.;Shenoy, Narmada;Shirasu, Ken;Sikhakolli, Usha R.;Stueber, Kurt;Sukno, Serenella A.;Sweigard, James A.;Takano, Yoshitaka;Takahara, Hiroyuki;Trail, Frances;van der Does, H. Charlotte;Voll, Lars M.;Will, Isa;Young, Sarah;Zeng, Qiandong;Zhang, Jingze;Zhou, Shiguo;Dickman, Martin B.;Schulze-Lefert, Paul;van Themaat, Emiel Ver Loren;Ma, Li-Jun;Vaillancourt, Lisa J.
通讯作者：
Vaillancourt, Lisa J.
影响因子：
4.4
作者：
Pedersen C;Ver Loren van Themaat E;McGuffin LJ;Abbott JC;Burgis TA;Barton G;Bindschedler LV;Lu X;Maekawa T;Wessling R;Cramer R;Thordal-Christensen H;Panstruga R;Spanu PD
通讯作者：
Spanu PD
DOI：
10.1073/pnas.0709303105
发表时间：
2008-03-25
影响因子：
11.1
作者：
Jiang, Rays H. Y.;Tripathy, Sucheta;Tyler, Brett M.
通讯作者：
Tyler, Brett M.
影响因子：
2.8
作者：
Chen, Hongxin;Kovalchuk, Andriy;Asiegbu, Fred O.
通讯作者：
Asiegbu, Fred O.