Lifestyle transitions in plant pathogenic Colletotrichum fungi deciphered by genome and transcriptome analyses.
Lifestyle transitions in plant pathogenic Colletotrichum fungi deciphered by genome and transcriptome analyses.
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基因组和转录组分析的植物致病性真菌的生活方式过渡。
DOI：
10.1038/ng.2372
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发表时间：
2012-09
期刊：
影响因子：
30.8
通讯作者：
Vaillancourt, Lisa J.
中科院分区：
文献类型：
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作者：
O'Connell, Richard J.;Thon, Michael R.;Hacquard, Stephane;Amyotte, Stefan G.;Kleemann, Jochen;Torres, Maria F.;Damm, Ulrike;Buiate, Ester A.;Epstein, Lynn;Alkan, Noam;Altmueller, Janine;Alvarado-Balderrama, Lucia;Bauser, Christopher A.;Becker, Christian;Birren, Bruce W.;Chen, Zehua;Choi, Jaeyoung;Crouch, Jo Anne;Duvick, Jonathan P.;Farman, Mark A.;Gan, Pamela;Heiman, David;Henrissat, Bernard;Howard, Richard J.;Kabbage, Mehdi;Koch, Christian;Kracher, Barbara;Kubo, Yasuyuki;Law, Audrey D.;Lebrun, Marc-Henri;Lee, Yong-Hwan;Miyara, Itay;Moore, Neil;Neumann, Ulla;Nordstroem, Karl;Panaccione, Daniel G.;Panstruga, Ralph;Place, Michael;Proctor, Robert H.;Prusky, Dov;Rech, Gabriel;Reinhardt, Richard;Rollins, Jeffrey A.;Rounsley, Steve;Schardl, Christopher L.;Schwartz, David C.;Shenoy, Narmada;Shirasu, Ken;Sikhakolli, Usha R.;Stueber, Kurt;Sukno, Serenella A.;Sweigard, James A.;Takano, Yoshitaka;Takahara, Hiroyuki;Trail, Frances;van der Does, H. Charlotte;Voll, Lars M.;Will, Isa;Young, Sarah;Zeng, Qiandong;Zhang, Jingze;Zhou, Shiguo;Dickman, Martin B.;Schulze-Lefert, Paul;van Themaat, Emiel Ver Loren;Ma, Li-Jun;Vaillancourt, Lisa J.
Richard O 'Connell及其同事报告了两种炭疽菌属植物真菌病原体的基因组和转录组。C. higginsianum侵染拟南芥，C. Graminicola感染玉米（玉米）;两个物种的比较基因组学导致从活体营养到坏死营养生命阶段的转变的分子见解。本文的在线版本（doi：10.1038/ng.2372）包含补充材料，可供授权用户使用。炭疽菌属（Colletotrichum）是一种在世界范围内危害农作物的真菌病原菌。宿主感染涉及特化细胞类型的分化，其与活宿主细胞内的渗透、生长（活体营养）和组织破坏（坏死营养）相关。我们在这里报告的基因组和转录组分析的Colletotrichum higginsianum感染拟南芥和Colletotrichum graminicola感染玉米。比较基因组学表明，这两种真菌都具有大量的致病性相关基因，但编码分泌效应子、果胶降解酶、次级代谢酶、转运蛋白和肽酶的基因家族在C. Higginsianum。全基因组表达谱分析表明，这些基因在连续波中转录，这些波与致病性转变有关：效应子和次级代谢酶在穿透前和活体营养期间被诱导，而大多数水解酶和转运蛋白随后在切换到坏死营养时被上调。我们的研究结果表明，植物来源的信号的入侵前的感知实质上重新编程真菌基因的表达，并表明以前未知的功能，为特定的真菌细胞类型。本文的在线版本（doi：10.1038/ng.2372）包含补充材料，可供授权用户使用。
Richard O'Connell and colleagues report the genomes and transcriptomes of two Colletotrichum plant fungal pathogens. C. higginsianum infects Arabidopsis thaliana, and C. graminicola infects maize (Zea mays); comparative genomics in both species lead to molecular insights into the transition from biotrophic to necrotrophic life stages. The online version of this article (doi:10.1038/ng.2372) contains supplementary material, which is available to authorized users. Colletotrichum species are fungal pathogens that devastate crop plants worldwide. Host infection involves the differentiation of specialized cell types that are associated with penetration, growth inside living host cells (biotrophy) and tissue destruction (necrotrophy). We report here genome and transcriptome analyses of Colletotrichum higginsianum infecting Arabidopsis thaliana and Colletotrichum graminicola infecting maize. Comparative genomics showed that both fungi have large sets of pathogenicity-related genes, but families of genes encoding secreted effectors, pectin-degrading enzymes, secondary metabolism enzymes, transporters and peptidases are expanded in C. higginsianum. Genome-wide expression profiling revealed that these genes are transcribed in successive waves that are linked to pathogenic transitions: effectors and secondary metabolism enzymes are induced before penetration and during biotrophy, whereas most hydrolases and transporters are upregulated later, at the switch to necrotrophy. Our findings show that preinvasion perception of plant-derived signals substantially reprograms fungal gene expression and indicate previously unknown functions for particular fungal cell types. The online version of this article (doi:10.1038/ng.2372) contains supplementary material, which is available to authorized users.
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