HA/β-TCP Biphasic Calcium Phosphate Ceramics Derived From Butterfish Bones Loaded With Bone Marrow Mesenchymal Stem Cells Promote Osteogenesis
HA/β-TCP Biphasic Calcium Phosphate Ceramics Derived From Butterfish Bones Loaded With Bone Marrow Mesenchymal Stem Cells Promote Osteogenesis
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HA/β-TCP 双相磷酸钙陶瓷源自装载骨髓间充质干细胞的鲳鱼骨，促进成骨
DOI：
10.3389/fmats.2022.928075
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发表时间：
2022-07
影响因子：
3.2
通讯作者：
Shaohai Wang
中科院分区：
文献类型：
--
作者：
Kehan Deng;Zhixiao Liu;Wenxufe Dou;Qi Cai;Wei Ma;Shaohai Wang
含有羟基磷灰石（HA）和β-磷酸三钙（β-TCP）的双相磷酸钙（BCP）被认为是骨修复的金标准。然而，嵌段共聚物合成的复杂性限制了其广泛应用。本课题前期工作证明了从渔业废弃物中提取的HA/β-TCP BCP陶瓷材料作为骨缺损填充的骨替代移植材料具有良好的前景。本研究旨在探讨蝴蝶鱼骨源性BCP材料在体内和体外的成骨作用。材料负载人骨髓间充质干细胞后，扫描电镜观察细胞粘附和存活情况。Western blot检测成骨细胞的表达。采用显微CT和苏木精-伊红染色检测大鼠股骨缺损模型的骨再生和材料降解率。结果显示hBMSCs生长良好，与材料贴附紧密。在体外，900°C煅烧的蝴蝶鱼骨的骨形成相关标志物ALP和Runx-2的表达水平普遍高于其他组。值得注意的是，在体内，900°C煅烧的蝴蝶鱼骨的成骨能力几乎与临床上最常用的Bio-Oss相当，并且明显优于其他组。综上所述，以蝴蝶鱼骨为原料制备的BCP陶瓷材料具有良好的生物相容性、骨传导性和骨诱导性，在骨移植替代材料领域具有良好的应用前景。
Biphasic calcium phosphate (BCP) containing hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP) is considered the gold standard for bone repair. However, the complex synthesis of BCP restricts the wide application of BCP. In the preliminary work, we proved that the HA/β-TCP BCP ceramic material extracted from fishery waste had a good prospect as a bone replacement graft material for filling bone defects. This study aimed to explore the osteogenesis effect of BCP material derived from butterfish bones in vivo and in vitro. After loading human bone marrow mesenchymal stem cells (hBMSCs) with materials, we used scanning electron microscopy to observe cell adhesion and survival. Western blot analysis was used to detect osteogenic expression in vitro. Micro-computed tomography and hematoxylin–eosin staining were used to detect bone regeneration and material degradation rate in the rat femoral defect model. The results showed that hBMSCs grew well and adhered closely to the material. In vitro, the expression levels of bone formation–related markers ALP and Runx-2 of butterfish bones calcined at 900°C were generally higher than those in the other groups. Notably, in vivo, the osteogenesis ability of butterfish bones calcined at 900°C was almost comparable to that of the most commonly used Bio-Oss in clinical practice and was significantly better than that in the other groups. In summary, the BCP ceramic material derived from butterfish bones had good biocompatibility, osteoconductivity, and osteoinductivity, and had a good application prospect in the field of bone graft substitutes.
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影响因子：
4.9
作者：
Dorozhkin SV
通讯作者：
Dorozhkin SV
基于间充质干细胞/前破骨细胞细胞外基质的工程支架促进骨再生
影响因子：
8.2
作者：
Dong, Rui;Bai, Yun;Dong, Shiwu
通讯作者：
Dong, Shiwu
DOI：
10.1016/j.msec.2012.08.014
发表时间：
2013-01-01
影响因子：
7.9
作者：
Piccirillo, C.;Silva, M. F.;Castro, P. M. L.
通讯作者：
Castro, P. M. L.
三种磷酸钙陶瓷（CaP）粉末诱导间充质干细胞（MSC）成骨分化的比较研究
DOI：
10.1016/j.msec.2017.05.145
发表时间：
2017-11-01
影响因子：
7.9
作者：
Li, Yuquan;Jiang, Tongmeng;Zhao, Jinmin
通讯作者：
Zhao, Jinmin
影响因子：
--
作者：
Dorozhkin, S. V.
通讯作者：
Dorozhkin, S. V.