Establishing a role for the GTPase Ypt1p at the late Golgi.
Establishing a role for the GTPase Ypt1p at the late Golgi.
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DOI：
10.1111/j.1600-0854.2010.01031.x
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发表时间：
2010-04
期刊：
影响因子：
--
通讯作者：
Ferro-Novick S
中科院分区：
文献类型：
--
作者：
Sclafani A;Chen S;Rivera-Molina F;Reinisch K;Novick P;Ferro-Novick S
Rab家族的GTP酶在非活性构象(GDP结合)和活跃构象(GTP结合)之间循环。RAB的活性形式通过多个效应器调节各种细胞功能。鸟嘌呤核苷酸交换因子(GEF)通过加速GDP向GTP的交换来激活RABS，而GTP酶激活蛋白(GAP)通过刺激GTP的水解来失活RABS。在酿酒酵母中，GTP酶Ypt1p是内质网高尔基体和高尔基体内运输所必需的。然而，最近的发现表明，Ypt1p全环、GAP和效应器都是从早期内体到高尔基体运输所必需的。在这里，我们描述了一个ypt1突变体的筛选，它阻止了从早期内吞体到晚期高尔基体的运输，但不能阻止一般的分泌。这一筛选导致了一组隐性和显性突变体的鉴定，这些突变体阻止了来自早期内体的运输。虽然人们早就知道Ypt1p调节生物合成的交通流进入高尔基体顺式侧，但这些发现确立了Ypt1p在调节早期内体-高尔基体交通中的作用。我们认为Ypt1p通过多个效应器调节进入高尔基体顺侧和横侧的交通流。
GTPases of the Rab family cycle between an inactive (GDP-bound) and active (GTP-bound) conformation. The active form of the Rab regulates a variety of cellular functions via multiple effectors. Guanine nucleotide exchange factors (GEFs) activate Rabs by accelerating the exchange of GDP for GTP, while GTPase activating proteins (GAPs) inactivate Rabs by stimulating the hydrolysis of GTP. The GTPase Ypt1p is required for ER-Golgi and intra-Golgi traffic in the yeast Saccharomyces cerevisiae. Recent findings, however, have shown that a Ypt1p GEF, GAP and effector are all required for traffic from the early endosome to the Golgi. Here we describe a screen for ypt1 mutants that block traffic from the early endosome to the late Golgi, but not general secretion. This screen has led to the identification of a collection of recessive and dominant mutants that block traffic from the early endosome. While it has long been known that Ypt1p regulates the flow of biosynthetic traffic into the cis side of the Golgi, these findings have established a role for Ypt1p in the regulation of early endosome-Golgi traffic. We propose that Ypt1p regulates the flow of traffic into the cis and trans side of the Golgi via multiple effectors.
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DOI：
10.1083/jcb.153.1.47
发表时间：
2001-04-02
期刊：
The Journal of cell biology
影响因子：
--
作者：
Rossanese OW;Reinke CA;Bevis BJ;Hammond AT;Sears IB;O'Connor J;Glick BS
通讯作者：
Glick BS
影响因子：
64.5
作者：
PROTOPOPOV, V;GOVINDAN, B;GERST, JE
通讯作者：
GERST, JE
影响因子：
7.8
作者：
FRANZUSOFF, A;REDDING, K;SCHEKMAN, R
通讯作者：
SCHEKMAN, R
影响因子：
64.5
作者：
NOVICK, P;FIELD, C;SCHEKMAN, R
通讯作者：
SCHEKMAN, R
影响因子：
3.3
作者：
Du, LL;Novick, P
通讯作者：
Novick, P