Evolutionarily conserved morphogenetic movements at the vertebrate head-trunk interface coordinate the transport and assembly of hypopharyngeal structures.
Evolutionarily conserved morphogenetic movements at the vertebrate head-trunk interface coordinate the transport and assembly of hypopharyngeal structures.
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DOI：
10.1016/j.ydbio.2014.03.003
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发表时间：
2014-06-15
影响因子：
2.7
通讯作者：
Dietrich, Susanne
中科院分区：
文献类型：
--
作者：
Lours-Calet, Corinne;Alvares, Lucia E.;El-Hanfy, Amira S.;Gandesha, Saniel;Walters, Esther H.;Sobreira, Debora Rodrigues;Wotton, Karl R.;Jorge, Erika C.;Lawson, Jennifer A.;Lewis, A. Kelsey;Tada, Masazumi;Sharpe, Colin;Kardon, Gabrielle;Dietrich, Susanne
关键词：
Evolution of vertebrate developmental mechanismsHead-trunk interfaceMorphogenetic movementsOccipital lateral mesodermOccipital somitesOccipital ectodermOccipital neural crestHypobranchial/hypoglossal muscleMigratory muscle precursorsFloor of pharynxPharyngeal archesCircumpharyngeal routeZebrafishXenopusChickenMouse
The vertebrate head–trunk interface (occipital region) has been heavily remodelled during evolution, and its development is still poorly understood. In extant jawed vertebrates, this region provides muscle precursors for the throat and tongue (hypopharyngeal/hypobranchial/hypoglossal muscle precursors, HMP) that take a stereotype path rostrally along the pharynx and are thought to reach their target sites via active migration. Yet, this projection pattern emerged in jawless vertebrates before the evolution of migratory muscle precursors. This suggests that a so far elusive, more basic transport mechanism must have existed and may still be traceable today. Here we show for the first time that all occipital tissues participate in well-conserved cell movements. These cell movements are spearheaded by the occipital lateral mesoderm and ectoderm that split into two streams. The rostrally directed stream projects along the floor of the pharynx and reaches as far rostrally as the floor of the mandibular arch and outflow tract of the heart. Notably, this stream leads and engulfs the later emerging HMP, neural crest cells and hypoglossal nerve. When we (i) attempted to redirect hypobranchial/hypoglossal muscle precursors towards various attractants, (ii) placed non-migratory muscle precursors into the occipital environment or (iii) molecularly or (iv) genetically rendered muscle precursors non-migratory, they still followed the trajectory set by the occipital lateral mesoderm and ectoderm. Thus, we have discovered evolutionarily conserved morphogenetic movements, driven by the occipital lateral mesoderm and ectoderm, that ensure cell transport and organ assembly at the head–trunk interface. At the vertebrate head–trunk interface, all tissues engage in stereotype cell movements. A ventrally–rostrally directed stream of cells leads along the floor of the pharynx to the developing jaw and outflow tract of the heart. The cell movements are spearheaded by the lateral mesoderm and surface ectoderm; muscle precursors for throat and tongue muscles (hypopharyngeal muscles); neural crest cells and outgrowing axons of the hypoglossal nerve follow. Hypopharyngeal muscle precursors follow the trajectory set by the lateral mesoderm and ectoderm, even when challenged with ectopic attractants or when rendered non-migratory. The newly discovered cell movements are the likely ground state for cell transport and organ assembly at the head–trunk interface before actively migrating muscle precursors evolved in “bony” (osteichthyan) vertebrates.
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影响因子：
2.7
作者：
Hosokawa R;Oka K;Yamaza T;Iwata J;Urata M;Xu X;Bringas P Jr;Nonaka K;Chai Y
通讯作者：
Chai Y
影响因子：
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作者：
Diogo R;Hinits Y;Hughes SM
通讯作者：
Hughes SM
影响因子：
2.4
作者：
Ainsworth, Sophie J.;Stanley, Rachael L.;Evans, Darrell J. R.
通讯作者：
Evans, Darrell J. R.
影响因子：
2.5
作者：
Bothe, Ingo;Dietrich, Susanne
通讯作者：
Dietrich, Susanne
影响因子：
2.7
作者：
Cheng, L;Alvares, LE;Dietrich, S
通讯作者：
Dietrich, S